[二氧化硅暴露大鼠的巨噬细胞凋亡及白细胞介素-1和白细胞介素-8水平]

[Macrophage apoptosis and the levels of interleukin-1 and interleukin-8 in the rats exposed to silica].

作者信息

Jin Yu-Lan, Zhang Wen-Li, Yao San-Qiao, Fan Xue-Yun, Xu Ying-Jun, Bai Yu-Ping, Yuan Ju-Xiang

机构信息

School of Public Health Hebei United University, Tangshan 063000 China.

出版信息

Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2011 Aug;29(8):562-6.

PMID:22335151

Abstract

OBJECTIVE

To study the roles of macrophage apoptosis, IL-1, and IL-8 in the pathogenesis of rat pulmonary fibrosis induced by silica.

METHODS

Forty eight male Wistar rats were divided into the 4 control groups (24 rats) and 4 experimental groups (24 rats). Rats in the control groups were treated with 1 ml normal saline by trachea instillation, whereas the rats in experimental groups were exposed 1 ml silica suspension (100 mg/ml) by trachea instillation for 1, 7, 14 and 28 days, respectively. Six rats of each group were sacrificed, then the bronchoalveolar lavage fluid and lung tissues were collected, respectively. Pulmonary inflammation, fibrosis and other pathological changes were detected with H.E. staining. Morphological changes of the early stage apoptosis in macrophages were detected with transmission electron microscope (TEM). The early apoptosis rates of macrophages in BALF were also assessed using Annexin V-FITC/PI kit. The IL-1 and IL-8 levels of serum were measured with the ELISA.

RESULTS

The apoptotic rates (11.48% +/- 0.24%, 16.03% +/- 0.68%, 15.53% +/- 1.07%, 18.92% +/- 2.70%, respectively) of macrophage in the experimental groups increased obviously with time, as compared to the controls (5.47% +/- 2.06%, 6.39% +/- 0.215, 9.07% +/- 0.61% and 8.54% +/- 0.16%, Respectively) (P < 0.05). The IL-1 levels of serum in the experimental groups were 23.64 +/- 0.84, 23.38 +/- 1.10, 22.21 +/- 0.86 and 24.29 +/- 1.31 pg/ml, respectively, which were significantly higher than those (18.52 +/- 1.23, 18.40 +/- 1.6, 17.92 +/- 2.21 and 18.53 +/- 2.64 pg/ml, respectively) in the control groups (P < 0.05) without time-effect relationship. The serum IL-8 levels on the 1st, 7th and 14th days in the experimental groups were 21.32 +/- 1.44, 21.90 +/- 2.08 and 22.00 +/- 2.80 pg/ml, respectively, which were significantly higher than those (17.69 +/- 1.09, 16.98 +/- 2.09 and 17.54 +/- 1.62 pg/ml, respectively) in the control groups (P < 0.05).

CONCLUSION

The early macrophage apoptosis and changes of IL-1 and IL-8 may in lungs may play an important role in the development of pulmonary fibrosis induced by silica.

摘要

目的

研究巨噬细胞凋亡、白细胞介素-1（IL-1）和白细胞介素-8（IL-8）在二氧化硅诱导的大鼠肺纤维化发病机制中的作用。

方法

48只雄性Wistar大鼠分为4个对照组（24只大鼠）和4个实验组（24只大鼠）。对照组大鼠经气管内滴注1 ml生理盐水，而实验组大鼠分别经气管内滴注1 ml二氧化硅悬浮液（100 mg/ml），持续1、7、14和28天。每组处死6只大鼠，然后分别收集支气管肺泡灌洗液和肺组织。用苏木精-伊红（H.E.）染色检测肺部炎症、纤维化及其他病理变化。用透射电子显微镜（TEM）检测巨噬细胞早期凋亡的形态学变化。还用膜联蛋白V-异硫氰酸荧光素/碘化丙啶（Annexin V-FITC/PI）试剂盒评估支气管肺泡灌洗液（BALF）中巨噬细胞的早期凋亡率。用酶联免疫吸附测定（ELISA）法检测血清中IL-1和IL-8水平。

结果

与对照组（分别为5.47%±2.06%、6.39%±0.215、9.07%±0.61%和8.54%±0.16%）相比，实验组巨噬细胞的凋亡率（分别为11.48%±0.24%、16.03%±0.68%、15.53%±1.07%、18.92%±2.70%）随时间明显增加（P<0.05）。实验组血清中IL-1水平分别为23.64±0.84、23.38±1.10、22.21±0.86和24.29±1.31 pg/ml，显著高于对照组（分别为18.52±1.23、18.40±1.6、17.92±2.21和18.53±2.64 pg/ml）（P<0.05），且无时间效应关系。实验组第1、7和14天血清中IL-8水平分别为21.32±1.44、21.90±2.08和22.00±2.80 pg/ml，显著高于对照组（分别为17.69±1.09、16.98±2.09和17.54±1.62 pg/ml）（P<0.05）。