4T1 鼠乳腺癌的传导热消融减少了存活肿瘤中的严重缺氧。
Conductive thermal ablation of 4T1 murine breast carcinoma reduces severe hypoxia in surviving tumour.
机构信息
Department of Radiation Oncology, University of Arkansas for Medical Sciences, Little Rock, Arkansas 72205, USA.
出版信息
Int J Hyperthermia. 2012;28(2):156-62. doi: 10.3109/02656736.2011.636783.
PURPOSE
The purpose of this study was to quantify hypoxia changes in viable tumour volumes after thermal ablation of a murine breast carcinoma.
METHODS
Murine breast 4T1 tumours were grown in the rear leg of BALB/c mice to an average diameter of 10-12 mm. Tumours were treated with conductive interstitial thermal therapy (CITT) at a peak temperature of 80-90°C for 10 min. The animals were euthanised 72 h later, and the tumours were removed for immunohistochemical staining with pimonidazole - a marker of partial pressure of oxygen. The levels of pimonidazole staining intensity were used to quantify changes in hypoxia gradients in terms of strong, medium and weak positive pixel fractions.
RESULTS
The pimonidazole staining ratio of viable control tumour tissue to viable tissue in tumours that were ablated was 0.7 for weak staining, 2.7 for medium staining and 8.0 (p < 0.03) for strong pimonidazole staining.
CONCLUSION
This shift of pimonidazole staining toward lower intensity pixels in the remaining tumour indicates that tumour ablation with CITT may increase radiosensitivity of the remaining tumour tissue and presents a rationale for combination therapy.
目的
本研究旨在量化热消融治疗后活肿瘤体积中的缺氧变化。
方法
将鼠乳腺癌 4T1 肿瘤种植于 BALB/c 小鼠后腿，肿瘤平均直径为 10-12mm。使用导电间质热疗（CITT），在 80-90°C 下治疗 10 分钟。72 小时后处死动物，切除肿瘤，用 pimonidazole（一种氧分压标志物）进行免疫组织化学染色。使用 pimonidazole 染色强度的水平来定量缺氧梯度的变化，分为强、中、弱阳性像素分数。
结果
活肿瘤组织与消融肿瘤组织中的活组织之间的 pimonidazole 染色比率，弱染色为 0.7，中染色为 2.7，强染色为 8.0（p < 0.03）。
结论
CITT 消融治疗后，剩余肿瘤中 pimonidazole 染色向低强度像素的转移表明，CITT 可能会增加剩余肿瘤组织的放射敏感性，并为联合治疗提供了理论依据。
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