Chiuini F, Della Torre G, Fanò G, Viti A
Acta Haematol. 1979;61(5):251-7. doi: 10.1159/000207668.
The effects of an erythropoietin (Ep)-rich plasma fraction and of a commercial preparation of purified Ep (Step I) on the cyclic adenosine monophosphate (cAMP) levels of suspended rabbit bone marrow cells at different incubation times (up to 10 min) were investigated. Both Ep preparations caused an early increase in cAMP concentration: using the plasma fraction a significant increase was found at the 6th, 7th, 9th and 10th min of incubation, whereas the purified Ep produces a significant increase at all incubation times, starting from 1 min. No effect was observed following incubation with fluorescein isothiocyanate (FITC)-inactivated Ep. These findings are discussed and possible role of cAMP as the intracellular messenger for Ep action is suggested.
研究了富含促红细胞生成素（Ep）的血浆组分和纯化Ep的商业制剂（第一步）在不同孵育时间（长达10分钟）对悬浮兔骨髓细胞中环磷酸腺苷（cAMP）水平的影响。两种Ep制剂均导致cAMP浓度早期升高：使用血浆组分时，在孵育的第6、7、9和10分钟发现显著升高，而纯化的Ep从1分钟开始在所有孵育时间均产生显著升高。用异硫氰酸荧光素（FITC）灭活的Ep孵育后未观察到效果。对这些发现进行了讨论，并提出了cAMP作为Ep作用的细胞内信使的可能作用。
