Ma Yu-qing, Zhang Chen, Cui Wen-li, Gulinaer Abulajiang, Zhang Wei, Wang Jian
Department of Pathology, Xinjiang Medical University, Urumqi, China.
Zhonghua Bing Li Xue Za Zhi. 2011 Dec;40(12):820-4.
To study the correlation between amplification of chromosome 1 and histological typing and clinical staging of thymic epithelial tumors according to the WHO classification.
Amplification of chromosome 1 was detected by interphase fluorescence in-situ hybridization (FISH) in 60 cases of thymic epithelial tumors, including type A thymoma (2 cases), type AB (19 cases), B1 (4 cases), B2 (14 cases), B3 (11 cases), metaplastic thymoma (2 cases), and thymic carcinoma (8 cases) and 11 samples of normal thymus.
Gain on chromosome 1 was found in 19 cases (31.7%) of thymic epithelial tumors, and none was detected in normal thymic tissues (P < 0.05). The positive rates of gain on chromosome 1 were statistically different among various histological subtypes of thymic epithelial tumors (P < 0.05), in which the highest rate of detection was in thymic carcinoma (6/8), the second, type B3 (6/11), followed by type A (1/2), type AB (4/19), type B2 (2/14) and type B1 (0). The positive rate of gain on chromosome 1 in type B3 had no statistical difference from thymic carcinoma (P > 0.05), but significantly higher than that in other types of thymoma (P < 0.05). In addition, the polysomy rate of chromosome 1 was significantly different among the thymic epithelial tumors at different clinical stages (P = 0.023), and that at stages III and IV was statistically higher than that in stages I and II (P = 0.003) but there was no significant difference between stage I and stage II tumors (P = 0.750).
Gain on chromosome 1 is more common in thymic carcinoma and type B3 thymoma than that in other subtypes of thymic epithelial tumors. Thymoma of type B3 may have different genetic features from other subtypes. Detection of gain on chromosome 1 by FISH is helpful in the differential diagnosis and prediction of prognosis in patients with thymic epithelium tumors.
根据世界卫生组织（WHO）分类，研究1号染色体扩增与胸腺上皮肿瘤组织学类型及临床分期之间的相关性。
采用间期荧光原位杂交（FISH）技术检测60例胸腺上皮肿瘤（包括A型胸腺瘤2例、AB型19例、B1型4例、B2型14例、B3型11例、化生型胸腺瘤2例、胸腺癌8例）及11例正常胸腺组织中1号染色体的扩增情况。
19例（31.7%）胸腺上皮肿瘤检测到1号染色体获得性改变，正常胸腺组织均未检测到（P<0.05）。胸腺上皮肿瘤不同组织学亚型中1号染色体获得性改变的阳性率差异有统计学意义（P<0.05），其中胸腺癌最高（6/8），其次为B3型（6/11），A型（1/2）、AB型（4/19）、B2型（2/14）、B1型（0）依次降低。B3型1号染色体获得性改变阳性率与胸腺癌差异无统计学意义（P>0.05），但显著高于其他类型胸腺瘤（P<0.05）。此外，不同临床分期的胸腺上皮肿瘤1号染色体多体率差异有统计学意义（P=0.023），Ⅲ期和Ⅳ期显著高于Ⅰ期和Ⅱ期（P=0.003），但Ⅰ期与Ⅱ期肿瘤间差异无统计学意义（P=0.750）。
1号染色体获得性改变在胸腺癌和B3型胸腺瘤中比在其他胸腺上皮肿瘤亚型中更常见。B3型胸腺瘤可能具有与其他亚型不同的遗传特征。FISH检测1号染色体获得性改变有助于胸腺上皮肿瘤患者的鉴别诊断和预后预测。
