Enochson Lars, Sandstedt Joakim, Strandberg Hans, Emanuelsson Cecilia, Ornberg Andreas, Lindahl Anders, Karlsson Camilla
Department of Clinical Chemistry and Transfusion Medicine, The Sahlgrenska Academy, Gothenburg University, Gothenburg, Sweden.
J Electrocardiol. 2012 May-Jun;45(3):305-11. doi: 10.1016/j.jelectrocard.2012.01.004. Epub 2012 Feb 14.
Within pacemaker research few attempts have been made to find an optimal waveform phase sequence that synchronizes beating of cardiomyocytes at an electrode. Multielectrode arrays (MEAs) offer electrophysiological screening of cardiomyocytes serving as a system for preliminary screening of pacing waveform design.
The HL-1 cell line was cultured in MEAs until confluence and stimulated with biphasic, triphasic, and quadriphasic waveforms. The amplitudes required for synchronized beating of the cells were determined.
Triphasic and quadriphasic waveforms were more efficient in eliciting synchronized beating of the HL-1 cells compared with the biphasic waveform because it allows significant reductions in synchronizing voltage amplitudes and reductions in supplied stimulus.
The MEA system allows for a straightforward manner to investigate effects of waveform design on synchronized beating in cardiomyocytes in vitro. Increased number of phase changes in a pacing waveform seems to be the major reason for the reduction in synchronizing amplitudes.
在起搏器研究中，很少有人尝试寻找一种能使电极处心肌细胞搏动同步的最佳波形相位序列。多电极阵列（MEA）可对心肌细胞进行电生理筛选，作为起搏波形设计初步筛选的系统。
将HL-1细胞系培养在MEA中直至汇合，并用双相、三相和四相波形进行刺激。确定细胞同步搏动所需的振幅。
与双相波形相比，三相和四相波形在引发HL-1细胞同步搏动方面更有效，因为它能显著降低同步电压振幅并减少所提供的刺激。
MEA系统提供了一种直接的方式来研究波形设计对体外心肌细胞同步搏动的影响。起搏波形中相位变化数量的增加似乎是同步振幅降低的主要原因。
