Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Research Center for Nanobiology and Nanomedicine (MOE 985 Innovative Platform), Wuhan University, Wuhan, 430072, PR China.
Nanoscale. 2012 Mar 21;4(6):2054-9. doi: 10.1039/c2nr12061e. Epub 2012 Feb 15.
Mucin 1 (MUC1) which presents in epithelial malignancies, is a well-known tumor biomarker. In this paper, a highly sensitive and selective fluorescent aptasensor for Mucin 1 (MUC1) detection is constructed, utilizing graphene oxide (GO) as a quencher which can quench the fluorescence of single-stranded dye-labeled MUC1 specific aptamer. In the absence of MUC1, the adsorption of the dye-labeled aptamer on GO brings the dyes in close proximity to the GO surface resulting in high efficiency quenching of dye fluorescence. Therefore, the fluorescence of the designed aptasensor is completely quenched by GO, and the system shows very low background fluorescence. Conversely, and very importantly, upon the adding of MUC1, the quenched fluorescence is recovered significantly, and MUC1 can be detected in a wide range of 0.04-10 μM with a detection limit of 28 nM and good selectivity. Moreover, the results have also been verified for real sample application by testing 2% serum containing buffer solution spiked with a series of concentrations of MUC1.
黏蛋白 1（MUC1）在上皮性恶性肿瘤中表达，是一种众所周知的肿瘤标志物。在本文中，构建了一种用于检测黏蛋白 1（MUC1）的高灵敏度和选择性荧光适体传感器，利用氧化石墨烯（GO）作为猝灭剂，能够猝灭单链染料标记的 MUC1 特异性适体的荧光。在没有 MUC1 的情况下，染料标记的适体在 GO 上的吸附使染料靠近 GO 表面，从而实现染料荧光的高效猝灭。因此，设计的适体传感器的荧光完全被 GO 猝灭，系统显示非常低的背景荧光。相反，非常重要的是，当加入 MUC1 时，猝灭的荧光显著恢复，并且可以在 0.04-10 μM 的宽范围内检测到 MUC1，检测限为 28 nM，具有良好的选择性。此外，还通过测试含有 2%血清的缓冲溶液中一系列浓度的 MUC1 来验证实际样品应用的结果。
