Zhang Jun, Ran Fengying, Zhou Wenbo, Shang Bing, Yu Fei, Wu Lun, Hu Wanbao, He Xueqin, Chen Qinhua
Affiliated Dongfeng Hospital, Hubei University of Medicine Shiyan Hubei 442008 China
Sinopharm Dongfeng Huaguo Hospital Shiyan 442008 Hubei China.
RSC Adv. 2018 Sep 14;8(56):32009-32015. doi: 10.1039/c8ra06498a. eCollection 2018 Sep 12.
A novel sensing strategy for sensitive detection of mucin 1 protein (MUC1) based on deoxyribonuclease I-aided target recycling signal amplification was proposed. In this paper, in the absence of MUC1, the MUC1 aptamer is absorbed on the surface of graphene oxide (GO) π-stacking interactions. This results in quenching of the fluorescent label and no fluorescence signal is observed. Upon adding MUC1, the probe sequences could be specifically recognized by MUC1, leading to an increase in the fluorescence intensity. The detection limit is as low as 10 pg mL, and a linear range from 50 pg mL to 100 ng mL. The assay is specific and sensitive, and successfully applied to the determination of MUC1 in spiked human serum, urine and saliva. Importantly, the proposed aptasensing strategy has great potential in detecting various protein and even cancer cells.
提出了一种基于脱氧核糖核酸酶I辅助目标循环信号放大的新型传感策略，用于灵敏检测粘蛋白1（MUC1）。本文中，在不存在MUC1的情况下，MUC1适配体通过π-堆积相互作用吸附在氧化石墨烯（GO）表面。这导致荧光标记淬灭，未观察到荧光信号。加入MUC1后，探针序列可被MUC1特异性识别，导致荧光强度增加。检测限低至10 pg/mL，线性范围为50 pg/mL至100 ng/mL。该测定具有特异性和灵敏性，并成功应用于加标人血清、尿液和唾液中MUC1的测定。重要的是，所提出的适配体传感策略在检测各种蛋白质甚至癌细胞方面具有巨大潜力。
