Infectious and Tropical Disease Research Center, Ahwaz Jondi-Shapoor University of Medical Sciences, Ahwaz, Iran.
J Med Virol. 2012 Apr;84(4):582-6. doi: 10.1002/jmv.23238.
Hepatitis A virus (HAV) is one of the etiologic agents of acute viral hepatitis, an important public health problem worldwide. The aim of this study was to investigate the genetic diversity of HAV in Southwest Iran (Ahwaz). A total of 59 sera were collected from acutely ill patients with anti-HAV IgM antibodies during 2009 and 2010 were tested also by RT-PCR targeting the 5' NCR for molecular diagnosis and examined in the VP1-2A and VP3-VP1 regions for genotyping. Twelve (20%) patients were detected VP1-2A by RT-PCR and 10 patients had VP3-VP1. The resulting amplicons were sequenced for genotype identification. All HAV strains were identified as subgenotype IB. Phylogenetic analysis revealed an extensive genetic heterogeneity among the strains. Seven hundred sixty-five S→F and 788 K→R amino acid substitutions in IRI49 isolate were found. It is concluded that subgenotype 1b is the sole genotype HAV in this region.
甲型肝炎病毒（HAV）是引起急性病毒性肝炎的病原体之一，是全球范围内一个重要的公共卫生问题。本研究旨在调查伊朗西南部（阿瓦士）HAV 的遗传多样性。2009 年和 2010 年，共收集了 59 份来自急性抗-HAV IgM 抗体阳性患者的血清，采用针对 5'NCR 的 RT-PCR 进行分子诊断，并在 VP1-2A 和 VP3-VP1 区进行基因分型检测。12 名（20%）患者的 RT-PCR 检测到 VP1-2A，10 名患者的 VP3-VP1 检测到 VP1-2A。对扩增产物进行测序以确定基因型。所有 HAV 株均鉴定为亚基因型 1B。系统进化分析显示，株间存在广泛的遗传异质性。在 IRI49 分离株中发现了 765 个 S→F 和 788 个 K→R 氨基酸取代。研究结论认为，该地区唯一的 HAV 基因型是 1b 亚型。
