Ali Sanaa Ahmed
Department of Therapeutic Chemistry, National Research Centre, Dokki, Giza, Egypt.
J Infect Dev Ctries. 2012 Feb 13;6(2):166-75. doi: 10.3855/jidc.1549.
The influence of vaccination on healthy (non-infected) rabbits treated with Schistosoma mansoni egg antigens, cercariae, and worms as prophylactic agents against infection, and the benefit of beta alanyl-l-histidine treatment against Schistosoma mansoni infection were investigated.
This study involved individual injection of three Schistosoma mansoni antigens: soluble egg antigen (SEA), cercarial antigen preparation (CAP) and soluble worm antigen preparation (SWAP), in three rabbit groups, respectively. Three other groups each received the same specific antigen in conjunction with the administration of (beta alanyl-l-histidine) L-carnosine. Hepatic total protein, glycogen and glycogen phosphorylase, total serum protein and one diminution electrophoresis of protein fractions (180 KDa; 116 KDa; 97, 4 KDa, serum albumin 66 KDa; 48. 5 KDa; 29 KDa; 18.400 KDa; 14.200 KDa; 6.5 KDa) were measured in all the rabbit groups.
Elevation in most parameters was observed in the immunized groups. Carnosine treatment of rabbit groups immunized with SEA, CAP and SWAP in comparison to the non-carnosine-treated immunized groups resulted in amelioration of serum protein fractions in all SEA and SWAP-immunized animals, and reduction in glycogen phosphorylase b in SWAP animals alone. In addition, changes in glycogen content were observed in the CAP-immunized group.
L-carnosine has a beneficial effect in the amelioration of most biochemical parameters as a result of S. mansoni antigen immunization.
研究了接种疫苗对用曼氏血吸虫卵抗原、尾蚴和虫体作为预防感染的药物处理的健康（未感染）兔子的影响，以及β-丙氨酰-L-组氨酸处理对曼氏血吸虫感染的益处。
本研究分别对三组兔子单独注射三种曼氏血吸虫抗原：可溶性虫卵抗原（SEA）、尾蚴抗原制剂（CAP）和可溶性虫体抗原制剂（SWAP）。另外三组每组在接种相同特异性抗原的同时给予（β-丙氨酰-L-组氨酸）L-肌肽。测定了所有兔子组的肝脏总蛋白、糖原和糖原磷酸化酶、血清总蛋白以及蛋白质组分的一维电泳（180 kDa；116 kDa；97.4 kDa；血清白蛋白66 kDa；48.5 kDa；29 kDa；18.400 kDa；14.200 kDa；6.5 kDa）。
在免疫组中观察到大多数参数升高。与未用肌肽处理的免疫组相比，用SEA、CAP和SWAP免疫的兔子组经肌肽处理后，所有SEA和SWAP免疫动物的血清蛋白组分得到改善，仅SWAP免疫动物的糖原磷酸化酶b降低。此外，在CAP免疫组中观察到糖原含量的变化。
L-肌肽对曼氏血吸虫抗原免疫导致的大多数生化参数改善具有有益作用。
