Karran P, Stephenson C, Cairns-Smith S, Macpherson P
Imperial Cancer Research Fund, Clare Hall Laboratories, South Mimms, Herts., Great Britain.
Mutat Res. 1990 Nov-Dec;233(1-2):23-30. doi: 10.1016/0027-5107(90)90147-v.
We have investigated the expression of the DNA-repair enzyme O6-methylguanine-DNA methyltransferase in the Burkitt's lymphoma cell line Raji. An existing mutant Raji cell line which lacks thymidine kinase activity had previously been shown to be Mex- and to no longer express O6-methylguanine-DNA methyltransferase. We report here that in addition to the methyltransferase and thymidine kinase, a third enzyme with an unrelated function, galactokinase, is also not expressed in Raji cells. The control of thymidine kinase expression is post-transcriptional and it is possible that galactokinase and methyltransferase can share a common post-transcriptional regulation with thymidine kinase.
我们研究了DNA修复酶O6-甲基鸟嘌呤-DNA甲基转移酶在伯基特淋巴瘤细胞系Raji中的表达情况。先前已证明，现有的缺乏胸苷激酶活性的突变Raji细胞系是Mex-，且不再表达O6-甲基鸟嘌呤-DNA甲基转移酶。我们在此报告，除了甲基转移酶和胸苷激酶外，具有不相关功能的第三种酶——半乳糖激酶，在Raji细胞中也不表达。胸苷激酶表达的调控是转录后水平的，半乳糖激酶和甲基转移酶有可能与胸苷激酶共享一种共同的转录后调控机制。
