Wang Dan, Fu Rong, Ruan Er-Bao, Qu Wen, Liang Yong, Wang Hua-Quan, Wang Jun, Li Li-Juan, Liu Hui, Wang Hong-Lei, Zhang Tian, Liu Hong, Wu Yu-Hong, Xing Li-Min, Wang Guo-Jin, Wang Xiao-Ming, Song Jia, Guan Jing, Sha Zong-Hong
Department of Hematology and Oncology, Tianjin Medical University General Hospital, Tianjin 300052, China.
Zhonghua Xue Ye Xue Za Zhi. 2011 Aug;32(8):543-7.
To study the expressions of erythropoietin receptor (EPOR) and thrombopoietin receptor (TPOR) on CD34+ CD59- and CD34+ CD59+ bone marrow (BM) cells from patients with paroxysmal nocturnal hemoglobinuria (PNH).
(1) The expressions of EPOR and TPOR on CD34+ CD59- and CD34+ CD59- BM cells from 26 PNH patients and 16 normal controls were examined by flow cytometry (FCM). (2) The mRNA expression of the EPOR and the TPOR in BM mononuclear cells (BMMNC) from 25 PNH patients and 13 normal controls were examined by RT-PCR.
(1) The percentage of EPOR positive cells in PNH CD34+ CD59+ BMMNC [(30.67 +/- 18.30)%] was significantly higher than that in PNH CD34+ CD59- BMMNC [(8.05 +/- 3.51)%] (P < 0.01) and than that in control CD34+ CD59+ BMMNC [(8.24 +/- 6.51)%] (P < 0.01), but there was no obvious difference between the CD34+ CD59-BMMNC in PNH and CD34+ CD59+ BMMNC in control. (2) The percentage of TPOR positive cells in PNH CD34+ CD59+ BMMNC [(28.15 +/- 17.75)%] was significantly higher than that in PNH CD34+ CD59-BMMNC [(15.65 +/- 14.45)%] (P < 0.05) and than that in control CD34+ CD59+ BMMNC [(10.77 +/- .39)%] (P < 0.01), but there was no obvious difference between the CD34+ CD59- BMMNC in PNH and CD34+ CD59+ BMMNC in control. (3) There was no statistic difference in EPOR mRNA and TPOR mRNA expressions in BMMNCs between PNH patients group [(0.41 +/- 0.37) and (0.32 +/- 0.19), respectively] and control group [(0.47 +/- 0.33) and (0.40 +/- 0.29), respectively].
The expression of EPOR and TPOR of PNH patients on BM CD34+ CD59+ cells are significantly higher than those on BM CD34+ CD59- cells. The difference may be due to abnormal transcription of both receptor coding genes.
研究阵发性睡眠性血红蛋白尿（PNH）患者骨髓中CD34+CD59-和CD34+CD59+骨髓细胞上促红细胞生成素受体（EPOR）和血小板生成素受体（TPOR）的表达情况。
（1）采用流式细胞术（FCM）检测26例PNH患者和16例正常对照者骨髓中CD34+CD59-和CD34+CD59+骨髓细胞上EPOR和TPOR的表达。（2）采用逆转录聚合酶链反应（RT-PCR）检测25例PNH患者和13例正常对照者骨髓单个核细胞（BMMNC）中EPOR和TPOR的mRNA表达。
（1）PNH患者CD34+CD59+ BMMNC中EPOR阳性细胞百分比[（30.67±18.30）%]显著高于PNH患者CD34+CD59- BMMNC[（8.05±3.51）%]（P<0.01），也高于正常对照者CD34+CD59+ BMMNC[（8.24±6.51）%]（P<0.01），但PNH患者CD34+CD59- BMMNC与正常对照者CD34+CD59+ BMMNC之间无明显差异。（2）PNH患者CD34+CD59+ BMMNC中TPOR阳性细胞百分比[（28.15±17.75）%]显著高于PNH患者CD34+CD59- BMMNC[（15.65±14.45）%]（P<0.05），也高于正常对照者CD34+CD59+ BMMNC[（10.77±.39）%]（P<0.01），但PNH患者CD34+CD59- BMMNC与正常对照者CD34+CD59+ BMMNC之间无明显差异。（3）PNH患者组BMMNC中EPOR mRNA和TPOR mRNA表达[分别为（0.41±0.37）和（0.32±0.19）]与对照组[分别为（0.47±0.33）和（- 0.40±0.29）]相比，无统计学差异。
PNH患者骨髓CD34+CD59+细胞上EPOR和TPOR的表达显著高于骨髓CD34+CD59-细胞。这种差异可能是由于两种受体编码基因的转录异常所致。
