Ishii Y, Samejima Y, Saji F, Nomura T
Department of Radiation Biology, Faculty of Medicine, Osaka University, Japan.
Mutat Res. 1990 Oct;242(2):151-5. doi: 10.1016/0165-1218(90)90041-y.
Eggs from B6 x C3F1 female mice, which were fertilized in vitro with sperm from C3 x 101F1 male mice, were treated with synthetic surfactants, alcohol sulfate (AS) and linear alkylbenzene sulfonate (LAS), and natural soap for 1 h at the pronucleus stage, and then cultivated for 5 days. Eggs treated with AS or LAS at concentrations of less than 0.025% developed to the blastocyst stage as well as the untreated ones. At concentrations of AS or LAS higher than 0.03% no egg developed beyond the 1-cell stage. There appeared to be a threshold concentration between 0.025% and 0.03% of AS or LAS on the development of the mouse egg. However, natural soap had no effect on the development of the mouse egg up to 0.05%. When AS or LAS was applied to the culture medium throughout the cultivation of fertilized eggs for 5 days, there also appeared to be a threshold concentration between 0.01% and 0.025%, but not in the case of natural soap. The results provide additional support to our previous observations that AS and LAS can interrupt mouse pregnancy by killing fertilized eggs.
将B6×C3F1雌性小鼠的卵子与C3×101F1雄性小鼠的精子进行体外受精，在原核期用合成表面活性剂、醇硫酸盐（AS）和直链烷基苯磺酸盐（LAS）以及天然皂处理1小时，然后培养5天。用浓度低于0.025%的AS或LAS处理的卵子发育到囊胚期的情况与未处理的卵子相同。当AS或LAS浓度高于0.03%时，没有卵子发育超过1细胞期。AS或LAS对小鼠卵子发育的影响似乎存在一个介于0.025%和0.03%之间的阈值浓度。然而，高达0.05%的天然皂对小鼠卵子的发育没有影响。当在受精卵培养5天的整个过程中将AS或LAS应用于培养基时，似乎也存在一个介于0.01%和0.025%之间的阈值浓度，但天然皂的情况并非如此。这些结果为我们之前的观察提供了额外支持，即AS和LAS可通过杀死受精卵来中断小鼠妊娠。
