Gibson W, Garside L
Department of Pathology, University of Bristol Veterinary School, Langford, U.K.
Mol Biochem Parasitol. 1990 Aug;42(1):45-53. doi: 10.1016/0166-6851(90)90111-x.
We have examined the inheritance of kinetoplast DNA (kDNA) in gentic crosses of trypanosomes. In 2 independent crosses of Trypanosoma brucei spp. trypanosomes, the kDNA maxicircles which carry the genes for mitochondrial biogenesis, were inherited from one parent only, as already found by other workers. However, the other component of kDNA, the minicircles, were inherited from both parents. This was demonstrated by Southern analysis using cloned minicircle probes. The inheritance of kDNA is therefore not uniparental. Our data point to fusion of the parental kinetoplast DNA networks during genetic exchange, with gradual loss of one or other parental maxicircle type due to random segregation of maxicircles at subsequent mitotic divisions. We infer that the first event of genetic exchange is fusion of parental trypanosomes (either haploid or diploid), followed at some point by fusion of the parental mitochondria.
我们研究了锥虫遗传杂交中动质体DNA（kDNA）的遗传情况。在布氏锥虫属锥虫的2个独立杂交实验中，携带线粒体生物发生相关基因的kDNA大环仅从一个亲本遗传而来，这与其他研究者的发现一致。然而，kDNA的另一个组分，即小环，则是从双亲遗传而来。这通过使用克隆的小环探针进行Southern分析得以证明。因此，kDNA的遗传并非单亲遗传。我们的数据表明，在遗传交换过程中亲本动质体DNA网络发生融合，由于在随后的有丝分裂中大环随机分离，一种或另一种亲本大环类型会逐渐丢失。我们推断，遗传交换的首个事件是亲本锥虫（单倍体或二倍体）的融合，随后在某个时间点是亲本线粒体的融合。
