Buchanan F C, Pillai J S
Department of Microbiology, University of Otago, Dunedin, New Zealand.
Mycopathologia. 1990 Jul;111(1):25-32. doi: 10.1007/BF02277297.
The germination of sporangia in Coelomomyces psorophorae var tasmaniensis (C. p. tas.) is uncoordinated and thus there is a mixture of developmental stages in any given population. Continuous urografin gradients separated out the critical stages of germinating sporangia giving four bands, each band representing a consecutive stage of germination. These stages were investigated for changes in the sporangial wall using Transmission Electron Microscopy (TEM). The sporangia have a typical two-layered wall, an electron dense outer layer which can be divided into three distinct sub-layers D1, D2, and D3 and an inner electron transparent secondary wall. Stage 3 sporangia have an intact D1 layer on their outer wall. In the subsequent stages (4 & 4b) there is a progressive breakdown of this layer.
塔斯马尼亚库蠓变种(Coelomomyces psorophorae var tasmaniensis,C. p. tas.)的孢子囊萌发不协调,因此在任何给定群体中都存在发育阶段的混合。连续的泛影葡胺梯度分离出发芽孢子囊的关键阶段,产生四条带,每条带代表萌发的一个连续阶段。使用透射电子显微镜(TEM)研究了这些阶段孢子囊壁的变化。孢子囊有一个典型的双层壁,一个电子致密的外层,可分为三个不同的子层D1、D2和D3,以及一个内部电子透明的次生壁。第3阶段的孢子囊外壁有完整的D1层。在随后的阶段(4和4b),该层逐渐分解。