[磷脂酰丝氨酸在正常血细胞上的表达及促凝血活性]

[Expression and procoagulant activity of phosphatidylserine on the normal blood cells].

作者信息

Liu Shu-chuan, Zhou Jin, Shi Jia-lan, Su Yan-hua

机构信息

Department of Hematology, the First Affiliated Hospital of Harbin Medical University, China.

出版信息

Zhonghua Xue Ye Xue Za Zhi. 2011 Dec;32(12):858-62.

PMID:22339962

Abstract

OBJECTIVE

To investigate the expression and procoagulant activity of phosphatidylserine (PS) on the normal peripheral blood cells of adults.

METHODS

Normal peripheral blood samples were collected from 10 healthy volunteers (5 ml from each volunteer), platelets, neutrophils, lymphocytes and erythrocytes were isolated. The expression and procoagulant activity of PS on normal blood cells were identified by flow cytometry, inhibition test with lactadherin as PS probe and coagulation anticoagulant, respectively.

RESULTS

There was PS expression on a few normal blood cells (9.1%, 5.4%, 3.9% and 3.2% in platelets, neutrophils, lymphocytes and erythrocytes, respectively). The PS on these normal blood cells in vitro showed significant procoagulant activity. The plasma recalcification time was shortened by 47%, 36.5%, 25% and 12.5% by platelets, neutrophils, lymphocytes and erythrocytes, respectively; the formation of factor Xa (through both intrinsic and extrinsic pathways) and thrombin was also increased by 13% - 26% by platelets, neutrophils, lymphocytes and erythrocytes, respectively.

CONCLUSION

The PS on normal blood cells in vivo may play a crucial role in the coagulation cascade.

摘要

目的

研究磷脂酰丝氨酸（PS）在成人正常外周血细胞上的表达及促凝血活性。

方法

采集10名健康志愿者的正常外周血样本（每位志愿者采集5ml），分离出血小板、中性粒细胞、淋巴细胞和红细胞。分别采用流式细胞术、以乳凝集素作为PS探针的抑制试验以及凝血抗凝试验，鉴定PS在正常血细胞上的表达及促凝血活性。

结果

少数正常血细胞上存在PS表达（血小板、中性粒细胞、淋巴细胞和红细胞中PS的表达率分别为9.1%、5.4%、3.9%和3.2%）。这些正常血细胞上的PS在体外表现出显著的促凝血活性。血小板、中性粒细胞、淋巴细胞和红细胞分别使血浆复钙时间缩短了47%、36.5%、25%和12.5%；血小板、中性粒细胞、淋巴细胞和红细胞分别使Xa因子（通过内源性和外源性途径）和凝血酶的生成增加了13% - 26%。