Azevedo I, Moura D, Trendelenburg U
Laboratório de Farmacologia, Faculdade de Medicina, Porto, Portugal.
Naunyn Schmiedebergs Arch Pharmacol. 1990 Aug;342(2):245-8. doi: 10.1007/BF00166972.
Rat vasa deferentia were incubated with 0.2 mumol/l 3H-noradrenaline for 60 min and then washed out with amine-free solution for 100 min. Autoradiography then revealed a preferential labelling of the varicosities in the immediate vicinity of the surface of the tissue. However, when tissues were obtained from reserpine- and pargyline-pretreated rats (to block vesicular uptake and monoamine oxidase), 3H-noradrenaline was able to penetrate more deeply into the tissue. These differences are in accordance with the view that the autoradiographs reflect the 3H-noradrenaline concentration gradient (within the extracellular space) generated by the neuronal uptake of the 3H-amine; the concentration gradient is steeper (and the heterogeneity of labelling is more pronounced) in tissues with intact vesicular uptake and monoamine oxidase than in tissues in which these mechanisms had been inhibited.
将大鼠输精管与0.2 μmol/l的3H-去甲肾上腺素一起孵育60分钟，然后用无胺溶液冲洗100分钟。放射自显影显示，组织表面紧邻区域的曲张体有优先标记。然而，当从经利血平和帕吉林预处理（以阻断囊泡摄取和单胺氧化酶）的大鼠获取组织时，3H-去甲肾上腺素能够更深入地渗透到组织中。这些差异与放射自显影反映由3H-胺的神经元摄取产生的（细胞外空间内的）3H-去甲肾上腺素浓度梯度这一观点一致；与这些机制被抑制的组织相比，囊泡摄取和单胺氧化酶完整的组织中浓度梯度更陡（且标记的异质性更明显）。
