Servicio de Microbiología, Hospital Universitario 12 de Octubre, Madrid, Spain.
Enferm Infecc Microbiol Clin. 2013 Feb;31(2):97-9. doi: 10.1016/j.eimc.2012.01.003. Epub 2012 Feb 15.
To assess a new immunochromatography (ICT) test that detects glutamate dehydrogenase (GDH) antigen and Clostridium difficile toxin A/B simultaneously, and to propose an algorithm for the diagnosis of C. difficile infection (CDI) based on this test.
We analysed 970 stool samples. Discrepant results between GDH and toxin A/B were resolved using toxigenic culture as the reference.
This test enabled us to obtain a conclusive result in <30min in 93.8% of the samples. Among the discrepant results (GDH (+)/Toxin A/B (-)), 41.7% (25/60) were found to be toxigenic C. difficile by toxigenic culture.
This test has a high sensitivity and specificity for the diagnosis of CDI.
评估一种新的免疫层析（ICT）检测方法，该方法同时检测谷氨酸脱氢酶（GDH）抗原和艰难梭菌毒素 A/B，基于该检测方法提出一种艰难梭菌感染（CDI）的诊断算法。
我们分析了 970 份粪便样本。GDH 和毒素 A/B 检测结果不一致的样本采用产毒培养作为参考进行解决。
该检测方法使我们能够在 <30min 内获得 93.8%样本的明确结果。在不一致的结果（GDH(+) /毒素 A/B(-)）中，通过产毒培养发现 41.7%（25/60）为产毒艰难梭菌。
该检测方法对 CDI 的诊断具有较高的灵敏度和特异性。
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