Department of Comparative Biomedical Sciences, University of Teramo, Piazza A. Moro 45, 64100 Teramo, Italy.
Res Vet Sci. 2012 Oct;93(2):898-903. doi: 10.1016/j.rvsc.2012.01.013. Epub 2012 Feb 15.
Enzootic pneumonia by Mycoplasma hyopneumoniae and pleuropneumonia by Actinobacillus pleuropneumoniae are among the most common and economically relevant pulmonary diseases in swine herds. We herein investigated the activity and expression of 5-lipoxygenase (5-LOX) and cyclooxygenase-2 (COX-2) in healthy and diseased porcine lungs, by means of immunohistochemical, immunochemical and biochemical assays. Diseased lungs showed a significantly higher activity and expression of 5-LOX and COX-2 in a wide range of cell types, thus suggesting the likely involvement of both enzymes in the pathogenesis of bacterial porcine pneumonia. Consistently, increased enzyme activities were paralleled by increased leukotriene B(4) (LTB(4)), a 5-LOX product and prostaglandin E(2) (PGE(2)), a COX-2 product, content in diseased versus healthy lungs.
猪支原体肺炎和猪传染性胸膜肺炎是猪群中最常见和最具经济相关性的肺部疾病。本研究通过免疫组织化学、免疫化学和生化分析，调查了健康和患病猪肺中 5-脂氧合酶（5-LOX）和环氧化酶-2（COX-2）的活性和表达。患病肺组织中 5-LOX 和 COX-2 在多种细胞类型中的活性和表达显著升高，提示这两种酶可能参与了细菌性猪肺炎的发病机制。同样，酶活性的增加伴随着 5-LOX 产物白三烯 B4（LTB4）和 COX-2 产物前列腺素 E2（PGE2）在患病肺组织中与健康肺组织相比的含量增加。
