Swette Center for Environmental Biotechnology, The Biodesign Institute, Arizona State University, 1001 S. McAllister Ave., Tempe, AZ 85287, USA.
Bioresour Technol. 2012 Apr;110:130-4. doi: 10.1016/j.biortech.2012.01.146. Epub 2012 Feb 2.
Extracellular enzymes offer active catalysis for hydrolysis of organic solid wastes in anaerobic digestion. To evidence the quantitative significance of hydrolytic enzyme activities for major waste components, track studies of thermophilic and mesophilic anaerobic sequencing-batch reactors (TASBR and MASBR) were conducted using a co-substrate of real organic wastes. During 1day batch cycle, TASBR showed higher amylase activity for carbohydrate (46%), protease activity for proteins (270%), and lipase activity for lipids (19%) than MASBR. In particular, the track study of protease identified that thermophilic anaerobes degraded protein polymers much more rapidly. Results revealed that differences in enzyme activities eventually affected acidogenic and methanogenic performances. It was demonstrated that the superior nature of enzymatic capability at thermophilic condition led to successive high-rate acidogenesis and 32% higher CH(4) recovery. Consequently, these results evidence that the coupling thermophilic digestion with sequencing-batch operation is a viable option to promote enzymatic hydrolysis of organic particulates.
细胞外酶为厌氧消化中有机固体废弃物的水解提供了有效的催化作用。为了证明水解酶活性对主要废物成分的定量意义，使用实际有机废物的共底物进行了嗜热和中温厌氧序批式反应器（TASBR 和 MASBR）的跟踪研究。在 1 天批处理周期中，TASBR 对碳水化合物的淀粉酶活性（46%）、蛋白质的蛋白酶活性（270%）和脂质的脂肪酶活性（19%）均高于 MASBR。特别是蛋白酶的跟踪研究表明，嗜热厌氧菌对蛋白质聚合物的降解速度要快得多。结果表明，酶活性的差异最终会影响产酸和产甲烷性能。结果表明，在高温条件下具有优越的酶能力，可实现连续的高速产酸和 32%更高的 CH4 回收。因此，这些结果证明，将高温消化与序批式操作相结合是促进有机颗粒酶解的可行选择。
