Department of Agricultural Biotechnology and Plant Genomics and Breeding Institute, College of Agriculture and Life Sciences, Seoul National University, Seoul 151-921, Republic of Korea.
Virus Res. 2012 Apr;165(1):81-9. doi: 10.1016/j.virusres.2012.01.013. Epub 2012 Feb 8.
A previous study showed that both Grapevine Algerian latent virus (GALV) and Tomato bushy stunt virus (TBSV) systemically infect Nicotiana benthamiana, but GALV causes systemic infection whereas TBSV causes only local lesions in Chenopodium quinoa (C. quinoa). We recently isolated GALV strain Naju (GALV-N) from Limonium sinense and TBSV strain Sacheon (TBSV-S) from tomato. Both viruses belong to the genus Tombusvirus and have a similar genome organization. To identify determinants of systemic infection of GALV-N in C. quinoa in the current study, we generated infectious clones and capsid protein (CP)-deletion clones for the two viruses and confirmed that CP of GALV-N is required for systemic infection of C. quinoa due to its primary structural role in virus assembly. Through the use of chimeras, we identified a viral factor in addition to CP that contributes to systemic infection by GALV-N. Inactivation of the p19 demonstrated that host-specific activities of p19 are necessary for efficient systemic infection of C. quinoa by GALV-N. Our study is the first report to determine the viral factors required for systemic infection of GALV in C. quinoa.
先前的研究表明，葡萄阿尔及利亚潜病毒（GALV）和番茄丛矮病毒（TBSV）均可系统感染菾菜（Nicotiana benthamiana），但 GALV 引起系统感染，而 TBSV 仅引起藜（Chenopodium quinoa）局部病斑。我们最近从灯心草属 Limonium sinense 中分离出 GALV 株 Naju（GALV-N），从番茄中分离出 TBSV 株 Sacheon（TBSV-S）。两种病毒均属于 Tombusvirus 属，具有相似的基因组结构。为了鉴定 GALV-N 在藜中系统感染的决定因素，我们生成了两种病毒的感染性克隆和衣壳蛋白（CP）缺失克隆，并证实由于 CP 在病毒组装中的主要结构作用，GALV-N 的 CP 是藜系统感染所必需的。通过使用嵌合体，我们确定了除 CP 以外的另一个病毒因子，该因子有助于 GALV-N 的系统感染。p19 的失活表明，p19 的宿主特异性活性对于 GALV-N 对藜的有效系统感染是必要的。本研究首次报道了确定 GALV 在藜中系统感染所需的病毒因子。
