CSEM Centre Suisse d'Electronique et de Microtechnique SA, Neuchâtel, Switzerland.
Biosens Bioelectron. 2012 Apr 15;34(1):94-9. doi: 10.1016/j.bios.2012.01.033. Epub 2012 Feb 3.
An integrated platform for a very sensitive detection of cocaine based on a refractometric biosensor is demonstrated. The system uses a waveguide grating biosensor functionalized with a cocaine multivalent antigen-carrier protein conjugate. The immunoassay scheme consists of the competitive binding of cocaine-specific antibodies to the immobilized conjugates. A 1000-fold enhancement of the sensor's sensitivity is achieved when using gold conjugated monoclonal antibodies instead of free antibodies. Together with the optimization of the assay conditions, the setup is designed for a quick identification of narcotics using automated sampling. The results show that the presence of cocaine in a liquid sample could be identified down to a concentration of 0.7 nM within one minute. This value can be reduced even further when longer binding time is allowed (0.2 nM after 15 min). Application of the system to detection of narcotics at airport security control points is discussed.
本文展示了一种基于折光生物传感器的可卡因超灵敏检测的集成平台。该系统采用了一种波导光栅生物传感器，其表面修饰有可卡因多价抗原载体蛋白缀合物。免疫分析方案包括可卡因特异性抗体与固定化缀合物的竞争性结合。与使用游离抗体相比，使用金偶联单克隆抗体可将传感器的灵敏度提高 1000 倍。结合对分析条件的优化，该检测系统旨在通过自动化采样快速识别麻醉品。结果表明，在一分钟内可将液体样品中可卡因的浓度检测低至 0.7 nM，当允许更长的结合时间时（15 分钟后为 0.2 nM），检测下限还可进一步降低。本文还讨论了该系统在机场安检点检测麻醉品的应用。
