Centre for Bioactive Discovery, School of Science and Technology, University of New England, Armidale, NSW 2351, Australia.
J Ethnopharmacol. 2012 May 7;141(1):72-9. doi: 10.1016/j.jep.2012.02.002. Epub 2012 Feb 10.
Pterocarpus marsupium Roxb. (PM) is an Ayurvedic traditional medicine well known for its antidiabetic potential.
To fractionate the antidiabetic constituent(s) of the aqueous of extract of PM hardwood (PME).
Bio-assay methods including, insulin secretion from mouse pancreas and glucose uptake by mouse skeletal muscle, were used to determine and fractionate the antidiabetic activity of PME. Results obtained from the in vitro experiments were then verified by examining the effect of PME on glucose clearance in normoglycemic, non-diabetic sheep in vivo.
Exposure of mouse pancreatic and muscle tissues to PME stimulated the insulin secretion and glucose uptake, respectively, in a concentration-dependent manner. PME-mediated muscle glucose uptake was not potentiated in the presence of insulin indicating that PME acts via pathways which are utilized by insulin. Bio-assay-guided fractionation of PME yielded a high molecular weight fraction which had potent antidiabetic properties in vitro, and in in vivo.
Our findings, we believe for the first time, provide novel insights for the antidiabetic constituents of PM and demonstrate that a high molecular weight constituent(s) of PM has potent insulinotrophic and insulin-like properties.
紫檀（PM）是一种阿育吠陀传统药物，以其抗糖尿病潜力而闻名。
从 PM 硬木（PME）的水提取物中分离抗糖尿病成分。
采用生物测定方法，包括从小鼠胰腺中分泌胰岛素和小鼠骨骼肌摄取葡萄糖，以确定和分离 PME 的抗糖尿病活性。然后通过检查 PME 对正常血糖、非糖尿病绵羊体内葡萄糖清除率的影响，验证体外实验的结果。
PM 暴露于小鼠胰腺和肌肉组织中，以浓度依赖的方式刺激胰岛素分泌和葡萄糖摄取。PM 介导的肌肉葡萄糖摄取在胰岛素存在下没有增强，表明 PME 通过胰岛素利用的途径起作用。生物测定指导的 PME 分级分离得到了一种高分子量的分数，该分数在体外和体内均具有很强的抗糖尿病特性。
我们的研究结果首次为 PM 的抗糖尿病成分提供了新的见解，并表明 PM 的一种高分子量成分具有很强的胰岛素促生长和胰岛素样特性。
