Universidad Nacional de Colombia, Sede Medellin, School of Physics, Medellin, Colombia.
Opt Lett. 2012 Feb 15;37(4):752-4. doi: 10.1364/OL.37.000752.
We present an automatic procedure for 3D tracking of micrometer-sized particles with high-NA digital lensless holographic microscopy. The method uses a two-feature approach to search for the best focal planes and to distinguish particles from artifacts or other elements on the reconstructed stream of the holograms. A set of reconstructed images is axially projected onto a single image. From the projected image, the centers of mass of all the reconstructed elements are identified. Starting from the centers of mass, the morphology of the profile of the maximum intensity along the reconstruction direction allows for the distinguishing of particles from others elements. The method is tested with modeled holograms and applied to automatically track micrometer-sized bubbles in a sample of 4 mm3 of soda.
我们提出了一种用于高数值孔径数字无透镜全息显微镜中微米级粒子 3D 跟踪的自动方法。该方法使用双特征方法来搜索最佳焦面，并区分粒子与重建全息图流中的伪影或其他元素。一组重建图像沿轴向投影到单个图像上。从投影图像中，确定所有重建元素的质心。从质心开始，沿着重建方向的最大强度轮廓的形态可以区分粒子与其他元素。该方法通过模拟全息图进行了测试，并应用于自动跟踪苏打样品中 4mm3 的微尺度气泡。
