Lawson Health Research Institute Imaging Program, Rm E5-109, St Joseph's Hospital, 268 Grosvenor St, London, ON, Canada.
Contrast Media Mol Imaging. 2012 Jan-Feb;7(1):76-84. doi: 10.1002/cmmi.469.
A challenge with cardiac cell therapy is determining the location of cells relative to infarct tissue. As cells are viable following ¹¹¹In-labeling, and first-pass CT imaging can identify regions of myocardial infarction, we evaluated the feasibility of a SPECT/CT system to localize cells relative to infarcted myocardium in a canine model.
Ten canines underwent surgical ligation of the left-anterior-descending artery and endothelial progenitor cells labeled with ¹¹¹In-tropolone were transplanted endocardially or epicardially. SPECT/CT was performed on day of transplantation, 4 and 10 days post-transplantation. For each imaging session first-pass perfusion CT was performed to delineate the area of reduced perfusion. SPECT and first-pass CT images were fused and evaluated. Contrast-to-noise ratios (CNR) were calculated for ¹¹¹In-SPECT images to evaluate cell detection.
The zone of reduced perfusion was well delineated on first-pass perfusion CT in all canines. The ¹¹¹In signal was visualized within this zone in all cases. Analysis of the CNRs suggests that cells may be followed for 11 effective half-lives using the images from first-pass perfusion CT to provide the anatomic landmarks.
In the setting of an acute myocardial infarction SPECT/[first-pass perfusion CT] is an effective hybrid platform for the localization of cells in relation to the area of reduced blood flow.
心脏细胞治疗的一个挑战是确定细胞相对于梗塞组织的位置。由于细胞在¹¹¹In 标记后仍然存活，并且首次通过 CT 成像可以识别心肌梗塞区域，因此我们评估了 SPECT/CT 系统在犬模型中相对于梗塞心肌定位细胞的可行性。
10 只犬接受了左前降支的手术结扎，并用¹¹¹In-替罗酮标记的内皮祖细胞进行心内膜或心外膜移植。在移植当天、移植后 4 天和 10 天进行 SPECT/CT。对于每个成像阶段，首先进行首次通过灌注 CT 以描绘灌注减少的区域。融合 SPECT 和首次通过 CT 图像并进行评估。计算 ¹¹¹In-SPECT 图像的对比噪声比 (CNR) 以评估细胞检测。
所有犬的首次通过灌注 CT 都能很好地描绘出灌注减少区。在所有情况下，¹¹¹In 信号都在该区域内可见。CNR 的分析表明，使用首次通过灌注 CT 的图像可以跟踪细胞 11 个有效半衰期，以提供解剖学标志。
在急性心肌梗死的情况下，SPECT/[首次通过灌注 CT]是一种有效的混合平台，用于定位与血流减少区域相关的细胞。
