Ji Fang, Jin Li-Sha, Zeng Xiao-Ming, Zhang Xiu-Juan, Zhang Yan-Chun, Sun Yun-Xiao, Gao Li-Hua, He Hong, Rao Jun-Hua, Liu Xiao-Ming, Peng Bai-Lu
Guangdong Entomological Institute, Guangzhou, China.
Dongwuxue Yanjiu. 2012 Feb;33(1):79-84. doi: 10.3724/SP.J.1141.2012.01079.
To explore pathological alteration of T2DM in cynomolgus monkeys, gene expression profiles of peripheral blood leukocytes from spontaneous and diet-induced T2DM models was analyzed using quantitative real-time PCR. Among 36 T2DM associated genes tested, 19 genes (including G6PC, CCR2B, CTLA4) displayed a similar expression pattern in both spontaneous and diet-induced T2DM models and were significantly up-regulated or down-regulated compared to controls. Interestingly, expression abundance of all up-regulated genes in the diet-induced T2DM was stronger, although not significantly, than spontaneous models, indicating diet-induced T2DM in monkeys should be a reliable research model for changes in gene expression. The characteristic gene expression pattern obtained here may be useful for the clinical diagnosis of T2DM.
为探究食蟹猴2型糖尿病（T2DM）的病理改变，采用定量实时PCR分析了自发和饮食诱导T2DM模型外周血白细胞的基因表达谱。在检测的36个T2DM相关基因中，19个基因（包括G6PC、CCR2B、CTLA4）在自发和饮食诱导T2DM模型中表现出相似的表达模式，与对照组相比显著上调或下调。有趣的是，饮食诱导T2DM中所有上调基因的表达丰度虽不显著但比自发模型更强，表明饮食诱导的食蟹猴T2DM应是基因表达变化的可靠研究模型。此处获得的特征性基因表达模式可能有助于T2DM的临床诊断。
