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[恒河猴神经祖细胞玻璃体腔内移植至大鼠的技术]

[A technique of rhesus monkey neural progenitor cells intravitreal transplant to rats].

作者信息

Bian Hui, Fan Yao-Dong, Guo Li-Yun, Yu Hua-Lin

机构信息

Department of Minimally Invasive Neurosurgery, First Affiliated Hospital of Kunming Medical College, Kunming,China.

出版信息

Dongwuxue Yanjiu. 2012 Feb;33(1):85-8. doi: 10.3724/SP.J.1141.2012.01085.

Abstract

To investigate a simple and effective intraocular xenotransplant technique of rhesus monkey neural progenitor cells to rats, mechanical injury was induced in the rat's right retina. And the GFP-labeled rhesus monkey neural progenitor cells suspension was slowly injected into the vitreous space of the right injured and left control eye. Confocal image suggested that the xenografted cells survived in both the injured and control eye, meanwhile the cells integrated in the injured right retina. The results demonstrated that intravitreal xenotransplant could be adopted as a simple and reliable method.

摘要

为研究一种将恒河猴神经祖细胞移植到大鼠眼内的简单有效的异种移植技术,在大鼠右视网膜诱导机械损伤。然后将绿色荧光蛋白标记的恒河猴神经祖细胞悬液缓慢注入右损伤眼和左对照眼的玻璃体腔。共聚焦图像显示,移植的细胞在损伤眼和对照眼中均存活,同时这些细胞整合到受伤的右视网膜中。结果表明,玻璃体腔内异种移植可作为一种简单可靠的方法。

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