Denis M, Ghadirian E
Unité de Recherche Pulmonaire, Centre Hospitalier de l'Université de Sherbrooke, Sherbrooke, Québec; and Montreal General Hospital Research Institute, Montreal, Quebec.
Can J Infect Dis. 1993 Jan;4(1):38-42. doi: 10.1155/1993/954372.
The immunotherapeutic potential of interleukin-2 (IL-2), tumour necrosis factor alpha (TNFα) and interferon gamma (IFN-γ) administered by aerosol was examined on mice infected with Mycobacterium tuberculosis by the aerogenic route. Infection of balb/c mice with 10(4) colony forming units (cfu) of M tuberculosis led to death of all mice at day 35 post infection after progressive microbial growth in the lungs. Aerosolization of IL-2 (100 μg per mouse) did not promote an increase in resistance to tuberculosis, as seen by growth of M tuberculosis in the lungs. Administration of IFN-γ or TNFα (100 μg) by the aerosol route led to a significant reduction in microbial growth in the lungs and a 100% survival of infected mice at day 60. Similarly, aerosolization of TNFα and IFN-γ combined led to a very high degree of tuberculostatic activity in the lungs of infected animals, but not superior to that seen with either cytokine alone. Administration of similar amounts of cytokines by repeated intraperitoneal infusions led to a very marginal improvement in mouse resistance. These results suggest that localized cytokine administration may be beneficial in the treatment of lung diseases.
通过气溶胶途径给予白细胞介素-2（IL-2）、肿瘤坏死因子α（TNFα）和干扰素γ（IFN-γ）的免疫治疗潜力，在经空气传播途径感染结核分枝杆菌的小鼠身上进行了研究。用10⁴个结核分枝杆菌菌落形成单位（cfu）感染balb/c小鼠，在肺部微生物进行性生长后，感染后第35天所有小鼠死亡。如肺部结核分枝杆菌的生长情况所示，气溶胶化IL-2（每只小鼠100μg）并未促进对结核病抵抗力的增加。通过气溶胶途径给予IFN-γ或TNFα（100μg）导致肺部微生物生长显著减少，感染小鼠在第60天的存活率为100%。同样，TNFα和IFN-γ联合气溶胶化导致感染动物肺部具有非常高的抑菌活性，但并不优于单独使用任何一种细胞因子时的效果。通过重复腹腔内输注给予相似量的细胞因子，小鼠的抵抗力仅有非常微小 的改善。这些结果表明，局部给予细胞因子可能对肺部疾病的治疗有益。
