Department of Anatomy and Research Center for Tumor Immunology, Inje University College of Medicine, Busan 614-735, Korea.
Immune Netw. 2011 Dec;11(6):390-8. doi: 10.4110/in.2011.11.6.390. Epub 2011 Dec 31.
Epstein Barr virus (EBV) infected B cells are transformed into lymphoblastoid cell lines. Some researchers suggested some a few similarities between this process and carcinogenesis. We observed the expression of CD80 and CD86, co-stimulatory molecules on EBV-transformed B cells and changes of CD54 expression after stimulation of CD80 and CD86.
CD80 and CD86 were stimulated using anti-CD80 and anti-CD86 monoclonal antibodies. To assess apoptosis and surface protein expression, flow cytometric analysis was performed. Intracellular signal molecules were evaluated by RT-PCR and immunoblot. Morphology and localization of proteins were examined using inverted or confocal microscope.
Cross-linking of CD80 and CD86 induced apoptosis and interfered with proliferation of EBV-transformed B cells, and dispersion of clumped cells. We also examined that their stimulation induced ROS accumulation and reduced CD54 expression. Interestingly, we observed that CD80 and CD86 diminished the expression of CD54 in different methods. Both CD80 and CD86 down-regulated activation of focal adhesion kinase. CD80 stimulus inhibited CD54 expression through mainly RhoA inactivation, while CD86 down-regulated Ras and JNK phosphorylation.
These results suggest that co-stimulatory CD80 and CD86 molecules, expressed EBV-transformed B cells, may play a role in apoptosis and cell adhesion.
EB 病毒（EBV）感染的 B 细胞被转化为淋巴母细胞系。一些研究人员认为这个过程与致癌作用有一些相似之处。我们观察了 EBV 转化的 B 细胞上共刺激分子 CD80 和 CD86 的表达以及 CD80 和 CD86 刺激后 CD54 表达的变化。
使用抗 CD80 和抗 CD86 单克隆抗体刺激 CD80 和 CD86。通过流式细胞术分析评估细胞凋亡和表面蛋白表达。通过 RT-PCR 和免疫印迹评估细胞内信号分子。使用倒置或共聚焦显微镜检查蛋白质的形态和定位。
CD80 和 CD86 的交联诱导了 EBV 转化的 B 细胞凋亡和增殖干扰，并导致细胞聚集体的分散。我们还观察到，它们的刺激诱导 ROS 积累并降低 CD54 的表达。有趣的是，我们观察到 CD80 和 CD86 以不同的方式减少了 CD54 的表达。CD80 和 CD86 均下调粘着斑激酶的激活。CD80 刺激主要通过 RhoA 失活抑制 CD54 的表达，而 CD86 下调 Ras 和 JNK 磷酸化。
这些结果表明，表达 EBV 的 B 细胞上的共刺激分子 CD80 和 CD86 可能在细胞凋亡和细胞黏附中起作用。
