Department of Biomedical Laboratory Science, College of Health Sciences, Yonsei University at Wonju, Wonju 220-710, Korea.
Immune Netw. 2011 Dec;11(6):416-9. doi: 10.4110/in.2011.11.6.416. Epub 2011 Dec 31.
Dextran sodium sulfate (DSS) is a widely used chemical model for inflammatory bowel disease (IBD). It is thought that imbalances in the T helper (Th) cell subsets contribute to IBD. Recent studies suggest that the acute DSS-colitis model is polarized toward a Th1/Th17 profile based on RT-PCR analysis of colonic tissues. In the current study we determined whether colonic Th cells from DSS-colitis mice were skewed toward the Th17 profile. Mice were treated with 5% DSS for 7 days and colonic T cells isolated and examined for production of IFN-γ (Th1 cell), IL-4 (Th2 cell) and IL-17 (Th17 cell) by intracellular flow cytometry. We found that the percentage of colonic Th17 cells were similar to non-treated controls but the percentage of Th1 cells were elevated in DSS-colitis mice. These results suggest that in the acute DSS-colitis model the colonic Th cells exhibit a Th1 profile and not a Th17 profile.
葡聚糖硫酸钠（DSS）是一种广泛用于炎症性肠病（IBD）的化学模型药物。人们认为辅助性 T 细胞（Th）亚群的失衡导致了 IBD。最近的研究表明，基于结肠组织的 RT-PCR 分析，急性 DSS-结肠炎模型偏向于 Th1/Th17 表型。在本研究中，我们确定了 DSS-结肠炎小鼠结肠中的 Th 细胞是否偏向于 Th17 表型。用 5% DSS 处理小鼠 7 天，分离结肠 T 细胞，并通过细胞内流式细胞术检测 IFN-γ（Th1 细胞）、IL-4（Th2 细胞）和 IL-17（Th17 细胞）的产生。我们发现，DSS-结肠炎小鼠结肠中的 Th17 细胞百分比与未处理的对照组相似，但 Th1 细胞的百分比升高。这些结果表明，在急性 DSS-结肠炎模型中，结肠 Th 细胞表现出 Th1 表型，而不是 Th17 表型。
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