University of Murcia, Faculty of Biology, Department of Cell Biology and Histology, Fish Innate Immune System Group, Campus de Espinardo, Cartagena, Spain.
Benef Microbes. 2012 Mar 1;3(1):77-81. doi: 10.3920/BM2011.0028.
In the present study, a feeding trial was conducted to evaluate the effect of inulin and heat-inactivated Bacillus subtilis, single or combined, on several innate immune activities of gilthead seabream (Sparus aurata). Forty-eight specimens were randomly assigned to four dietary treatments: 0 (control), inulin (10 g/kg, prebiotic group), B. subtilis (10(7) cfu/g, probiotic group), or B. subtilis + inulin (10(7) cfu/g + 10 g/kg, synbiotic group). After two and four weeks, six fish of each group were sampled, with the main innate immune parameters (natural haemolytic complement activity, serum and leucocyte peroxidase, phagocytosis, respiratory burst, and cytotoxic activities) being determined. Inulin or heat-inactivated B. subtilis failed to significantly stimulate the innate immune parameters assayed, although some activities showed no significant increase through these treatments. A combination of inulin and B. subtilis resulted in an increase of such parameters, with the haemolytic complement activity being the only one significantly stimulated. To conclude, inulin and B. subtilis, when administered as a synbiotic, have a synergistic effect and enhance some innate immune parameters of gilthead seabream.
在本研究中，进行了一项饲养试验，以评估菊糖和热灭活枯草芽孢杆菌单独或联合使用对金头鲷（Sparus aurata）几种固有免疫活性的影响。将 48 个样本随机分配到四个饮食处理组中：0（对照组）、菊糖（10 g/kg，益生元组）、枯草芽孢杆菌（10(7)cfu/g，益生菌组）或枯草芽孢杆菌+菊糖（10(7)cfu/g+10 g/kg，合生元组）。在两周和四周后，从每组中采集 6 条鱼，测定主要固有免疫参数（天然溶血补体活性、血清和白细胞过氧化物酶、吞噬作用、呼吸爆发和细胞毒性活性）。菊糖或热灭活枯草芽孢杆菌未能显著刺激测定的固有免疫参数，尽管通过这些处理，一些活性没有显著增加。菊糖和枯草芽孢杆菌的组合导致这些参数增加，溶血补体活性是唯一显著刺激的参数。总之，作为合生元使用的菊糖和枯草芽孢杆菌具有协同作用，可增强金头鲷的某些固有免疫参数。
