Povarov L S, Grigorian G L, Isaeva N N, Suskina V I
Antibiotiki. 1979 Jul;24(7):488-93.
Hydrochloride of 14-(I-oxyl-2,2,6,6-tetramethylpiperidyl-4)-acetoxyrubomycin (spin-labeled rubomycin or SL-rubomycin) was prepared by interaction of hydrochloride of 14-bromrubomycin with potassium salt of I-oxyl-2,2,6,6-tetramethylpiperidyl-4-acetic acid. Its interaction with DNA and synthetic poly A and poly U polyribonucleotides was studied. The character of the EPR spectra was indicative of DNA binding with SL-rubomycin and forming a system with a high level of regularity similar to that of liquid crystals. The results of the study of the EPR spectra correlated with the model of rubomycin intercalation between the pairs of DNA bases and were indicative of water surrounding of the nitroxyl group of SL-rubomycin bound with DNA.
14 -（1 - 氧基 - 2,2,6,6 - 四甲基哌啶 - 4） - 乙酰氧基柔红霉素盐酸盐（自旋标记柔红霉素或SL - 柔红霉素）通过14 - 溴柔红霉素盐酸盐与1 - 氧基 - 2,2,6,6 - 四甲基哌啶 - 4 - 乙酸钾盐相互作用制备而成。研究了其与DNA以及合成的聚A和聚U多聚核苷酸的相互作用。电子顺磁共振（EPR）光谱特征表明DNA与SL - 柔红霉素结合，并形成了一个具有类似于液晶的高度规则性的体系。EPR光谱研究结果与柔红霉素在DNA碱基对之间嵌入的模型相关，并且表明与DNA结合的SL - 柔红霉素的硝酰基周围有水分子。
