Laboratory of Applied Microbiology, Biotechnology Research Center, Kunming University of Science and Technology, Chenggong Higher Education Mega Center, Kunming 650500, People's Republic of China.
Curr Microbiol. 2012 May;64(5):449-56. doi: 10.1007/s00284-012-0093-8. Epub 2012 Feb 18.
Members of group III histidine kinases from different filamentous fungi were previously shown to mediate osmoregulation and resistance to dicarboximide, phenylpyrrole and, aromatic hydrocarbon fungicides. In this study, we report the disruption of the gene encoding group III histidine kinase, AlHK1, in the economically important plant pathogen Alternaria longipes. The AlHK1 gene disruption had pleiotropic effects on this fungus. Besides the expected osmosensitivity and fungicides resistance, AlHK1 participated in the spore production process. In addition, the ΔAlHK1 strains had stronger aggressive ability to infect their host plant than that of their parental strain, the wild-type strain C-00, suggested that AlHK1 was involved in the pathogenicity of A. longipes and performed in this function by a negative manner. This is the first report to our knowledge.
先前的研究表明，来自不同丝状真菌的 III 组组氨酸激酶成员可以介导渗透压调节和对二羧酰胺、苯并吡咯和芳烃类杀真菌剂的抗性。在这项研究中，我们报道了经济上重要的植物病原体长蠕孢菌中编码 III 组组氨酸激酶的基因 AlHK1 的缺失。AlHK1 基因的缺失对该真菌有多种影响。除了预期的渗透压敏感性和抗真菌剂抗性外，AlHK1 还参与了孢子的产生过程。此外，与亲本菌株 C-00 相比，ΔAlHK1 菌株对其宿主植物的侵染能力更强，这表明 AlHK1 参与了长蠕孢菌的致病性，并且以负向方式发挥作用。据我们所知，这是首次报道。
