Division in Anatomy and Developmental Biology, Department of Oral Biology, Research Center for Orofacial Hard Tissue Regeneration, Brain Korea 21 Project, Oral Science Research Center, College of Dentistry, Yonsei University, 50 Yonsei-ro, Seodaemun-gu, Seoul, 120-752, Korea.
Histochem Cell Biol. 2012 Jun;137(6):791-800. doi: 10.1007/s00418-012-0930-7. Epub 2012 Feb 18.
Tooth morphogenesis is regulated by sequential and reciprocal interaction between oral epithelium and neural-crest-derived ectomesenchyme. The interaction is controlled by various signal molecules such as bone morphogenetic protein (BMP), Hedgehog, fibroblast growth factor (FGF), and Wnt. Zeb family is known as a transcription factor, which is essential for neural development and neural-crest-derived tissues, whereas the role of the Zeb family in tooth development remains unclear. Therefore, this study aimed to investigate the expression profiles of Zeb1 and Zeb2 during craniofacial development focusing on mesenchyme of palate, hair follicle, and tooth germ from E12.5 to E16.5. In addition, we examined the interaction between Zeb family and BMP4 during tooth development. Both Zeb1 and Zeb2 were expressed at mesenchyme of the palate, hair follicle, and tooth germ throughout the stages. In the case of tooth germ at the cap stage, the expression of Zeb1 and Zeb2 was lost in epithelium-separated dental mesenchyme. However, the expression of Zeb1 and Zeb2 in the dental mesenchyme was recovered by Bmp4 signaling via BMP4-soaked bead and tissue recombination. Our results suggest that Zeb1 and Zeb2, which were mediated by BMP4, play an important role in neural-crest-derived craniofacial organ morphogenesis, such as tooth development.
牙齿形态发生受口腔上皮和神经嵴衍生的中胚层之间的顺序和相互作用的调节。这种相互作用受各种信号分子的控制，如骨形态发生蛋白（BMP）、Hedgehog、成纤维细胞生长因子（FGF）和 Wnt。Zeb 家族是一种转录因子，对于神经发育和神经嵴衍生组织至关重要，而 Zeb 家族在牙齿发育中的作用尚不清楚。因此，本研究旨在研究 Zeb1 和 Zeb2 在颅面发育过程中的表达谱，重点是腭中胚层、毛囊和牙胚从 E12.5 到 E16.5。此外，我们还研究了 Zeb 家族与 BMP4 在牙齿发育过程中的相互作用。在整个发育阶段，Zeb1 和 Zeb2 在腭、毛囊和牙胚的中胚层中均有表达。在帽状期牙胚中，Zeb1 和 Zeb2 的表达在上皮分离的牙间质中丢失。然而，通过 BMP4 浸泡珠和组织重组，BMP4 信号介导的 Zeb1 和 Zeb2 的表达在牙间质中得到恢复。我们的结果表明，Zeb1 和 Zeb2 在 BMP4 的介导下，在神经嵴衍生的颅面器官形态发生中发挥重要作用，如牙齿发育。
