Holme Inger Bæksted, Brinch-Pedersen Henrik, Lange Mette, Holm Preben Bach
Department of Genetics and Biotechnology, Aarhus University, Research Centre Flakkebjerg, Slagelse, Denmark.
Methods Mol Biol. 2012;847:151-61. doi: 10.1007/978-1-61779-558-9_13.
Agrobacterium-mediated transformation of in vitro cultured barley ovules is an attractive alternative to well-established barley transformation methods of immature embryos. The ovule culture system can be used for transformation with and without selection and has successfully been used to transform cultivars other than Golden Promise indicating minor genotype dependency. The method allows for the rapid and direct generation of high-quality transgenic plants where the transgenes are stably expressed and show Mendelian inheritance in subsequent generations.
农杆菌介导的离体培养大麦胚珠转化是成熟的未成熟胚大麦转化方法的一种有吸引力的替代方法。胚珠培养系统可用于有选择和无选择的转化，并且已成功用于转化除金色承诺之外的其他品种，表明基因型依赖性较小。该方法允许快速直接产生高质量的转基因植物，其中转基因稳定表达并在后代中表现出孟德尔遗传。
