Wang E C, Borysiewicz L K, Weetman A P
Department of Medicine, Addenbrooke's Hospital, Cambridge, UK.
Methods Mol Biol. 1992;80:347-57. doi: 10.1385/0-89603-204-3:347.
Immunomagnetic beads are uniform, polymer particles coated with a polystyrene shell that provides both a smooth hydrophobic surface to facilitate physical absorption of molecules, such as antibodies, and surface hydroxyl groups that allow covalent chemical binding of other bioreactive molecules, such as streptavidin, lectins, and peptides. Iron (III) oxide (Fe(2)O(3)) deposited in the core gives the beads superparamagnetic properties that lead to consistent and reproducible reactions to a magnetic field without permanent magnetization of the particles. These are the two qualities on which immunomagnetic separation (IMS) depends.
免疫磁珠是均匀的聚合物颗粒，表面包覆有聚苯乙烯壳，该壳既提供了光滑的疏水表面以促进分子（如抗体）的物理吸附，又提供了表面羟基，可实现其他生物活性分子（如链霉亲和素、凝集素和肽）的共价化学结合。沉积在核心的三氧化二铁（Fe₂O₃）赋予磁珠超顺磁性，使其在磁场中产生一致且可重复的反应，而颗粒不会永久磁化。这就是免疫磁珠分离（IMS）所依赖的两个特性。
