The David and Alice Jurist Institute for Research, Hackensack University Medical Center, Hackensack, NJ, USA.
In Vivo. 2012 Mar-Apr;26(2):207-11.
While cervical cancer incidence and mortality rates have declined in the United States, this cancer represents a worldwide threat. Human papilloma viral infection causes cervical neoplasia (CIN). 3,3'-Diindolylmethane (DIM) prevents or inhibits the progression from cervical dysplasia to cancer. The aim of this study is to determine the most effective dose of DIM given continuously in food, that significantly increases serum interferon gamma levels (IFN-γ) in the K14-HPV16 transgenic mouse model for cervical cancer.
Five doses of DIM in food were administered to the mouse model for 20 weeks. Serum Interferon gamma (IFN-γ) levels and estrogen metabolite levels were quantified.
At 1000 ppm DIM, serum IFN-γ concentrations were significantly increased (p<0.0396). The estrogen metabolites were unchanged. IFN-γ concentrations in CIN free mice and the percentage of CIN free transgenic mice were well correlated (r=0.88).
Significant increases in IFN-γ serum concentrations that correlate with the percentage of CIN free mice in each group indicate that 1000 ppm of DIM in food may be the most effective dose for future studies. These results may eventually lead to new and effective vaccination strategies in women already infected with the human papilloma virus.
虽然宫颈癌在美国的发病率和死亡率已经下降，但这种癌症仍是全球威胁。人乳头瘤病毒感染会导致宫颈癌前病变（CIN）。3,3'-二吲哚甲烷（DIM）可预防或抑制宫颈癌前病变进展为癌症。本研究旨在确定在食物中连续给予 DIM 的最有效剂量，这种剂量能显著增加宫颈癌 K14-HPV16 转基因小鼠模型的血清干扰素γ水平（IFN-γ）。
5 种剂量的 DIM 被给予该小鼠模型 20 周。血清干扰素γ（IFN-γ）水平和雌激素代谢物水平被定量。
在 1000ppm 的 DIM 时，血清 IFN-γ 浓度显著增加（p<0.0396）。雌激素代谢物未改变。CIN 阴性转基因小鼠的 IFN-γ 浓度与 CIN 阴性转基因小鼠的百分比呈良好相关性（r=0.88）。
血清 IFN-γ 浓度的显著增加与每组 CIN 阴性小鼠的百分比相关，这表明食物中 1000ppm 的 DIM 可能是未来研究的最有效剂量。这些结果可能最终导致针对已感染人乳头瘤病毒的女性的新型有效疫苗接种策略。
Zotero 插件