Department of Immunology, Genetics and Pathology, Rudbeck Laboratory, Uppsala University, Uppsala, Sweden.
Dev Dyn. 2012 Apr;241(4):770-86. doi: 10.1002/dvdy.23753. Epub 2012 Feb 21.
Angiogenesis is implicated in many pathological conditions. The role of the proteins involved remains largely unknown, and few vascular-specific drug targets have been discovered. Previously, in a screen for angiogenesis regulators, we identified Paladin (mouse: X99384, human: KIAA1274), a protein containing predicted S/T/Y phosphatase domains.
We present a mouse knockout allele for Paladin with a β-galactosidase reporter, which in combination with Paladin antibodies demonstrate that Paladin is expressed in the vasculature. During mouse embryogenesis, Paladin is primarily expressed in capillary and venous endothelial cells. In adult mice Paladin is predominantly expressed in arterial pericytes and vascular smooth muscle cells. Paladin also displays vascular-restricted expression in human brain, astrocytomas, and glioblastomas.
Paladin, a novel putative phosphatase, displays a dynamic expression pattern in the vasculature. During embryonic stages it is broadly expressed in endothelial cells, while in the adult it is selectively expressed in arterial smooth muscle cells.
血管生成与许多病理状况有关。涉及的蛋白质的作用在很大程度上尚不清楚，并且发现的血管特异性药物靶标很少。以前，在筛选血管生成调节剂时，我们鉴定了Paladin（小鼠：X99384，人：KIAA1274），一种含有预测的 S/T/Y 磷酸酶结构域的蛋白质。
我们提供了带有β-半乳糖苷酶报告基因的 Paladin 小鼠敲除等位基因，该基因与 Paladin 抗体结合使用，证明 Paladin 在脉管系统中表达。在小鼠胚胎发生过程中，Paladin 主要在毛细血管和静脉内皮细胞中表达。在成年小鼠中，Paladin 主要在动脉周细胞和血管平滑肌细胞中表达。Paladin 在人类大脑、星形细胞瘤和多形性胶质母细胞瘤中也表现出血管特异性表达。
Paladin 是一种新型假定的磷酸酶，在脉管系统中表现出动态表达模式。在胚胎期，它广泛表达于内皮细胞，而在成年期则选择性地表达于动脉平滑肌细胞。
