Krejsek J, Mandak J, Kunes P, Lonsky V, Kolackova M, Jankovicova K, Andrys C
Department of Clinical Immunology and Allergology, Charles University in Prague, Faculty of Medicine and University Hospital in Hradec Kralove, Czech Republic.
Perfusion. 2012 Jul;27(4):284-91. doi: 10.1177/0267659112439595. Epub 2012 Feb 21.
We evaluated the influence of methylprednisolone in cardiopulmonary bypass fluid on scavenger receptor for hemoglobin CD163 molecule expression on monocytes of patients who underwent elective coronary artery bypass grafting with cardiopulmonary bypass with either exposure to methylprednisolone present in the cardiopulmonary bypass fluid (20 patients), or without methylprednisolone in the cardiopulmonary bypass fluid (22 patients) and operated on without cardiopulmonary bypass (42 patients). The dynamics of CD163 expression was also followed in patients operated on without cardiopulmonary bypass. This study was a retrospective analysis of a comparison of two studies. The expression of CD163 was determined quantitatively by standardized flow cytometry technique. The similarities in the dynamics of CD163 monocyte expression, comparing the patients operated on with or without cardiopulmonary bypass, were found. Compared to the preoperative level, CD163 monocyte expression was significantly elevated on the 1(st) postoperative day. Monocyte CD163 expression on the 1(st) postoperative day was evidently similar in both groups of patients operated without cardiopulmonary bypass (median value of mean fluorescence intensity (MFI) 18,896; interquartile range from 27,538 to 57,711; median value of MFI 18,863; interquartile range from 16,514 to 26,559; n.s.), suggesting high reproducibility of our flow cytometric method; the monocyte CD163 expression was significantly higher (median value of MFI 37,902; interquartile range from 27,538 to 57,711) on the 1(st) postoperative day in patients exposed to methylprednisolone compared to patients without this exposure (median value of MFI 20,995; interquartile range from 16,321 to 29,623) (p<0.001). We concluded that the expression of hemoglobin scavenger receptor CD163 on monocytes of cardiac surgical patients is induced by methylprednisolone present in cardiopulmonary bypass fluid.
我们评估了体外循环液中的甲泼尼龙对接受择期冠状动脉搭桥术并行体外循环患者单核细胞上血红蛋白清除受体CD163分子表达的影响，这些患者分为两组，一组体外循环液中含有甲泼尼龙（20例患者），另一组体外循环液中不含甲泼尼龙（22例患者），还有一组未进行体外循环手术（42例患者）。同时也对未进行体外循环手术患者的CD163表达动态变化进行了跟踪。本研究是对两项研究比较的回顾性分析。采用标准化流式细胞术技术定量测定CD163的表达。比较了进行或未进行体外循环手术患者CD163单核细胞表达动态变化的相似性。与术前水平相比，术后第1天CD163单核细胞表达显著升高。两组未进行体外循环手术的患者术后第1天单核细胞CD163表达明显相似（平均荧光强度（MFI）中位数为18,896；四分位间距为27,538至57,711；MFI中位数为18,863；四分位间距为16,514至26,559；无显著性差异），表明我们的流式细胞术方法具有高重复性；与未接触甲泼尼龙的患者相比，接触甲泼尼龙的患者术后第1天单核细胞CD163表达显著更高（MFI中位数为37,902；四分位间距为27,538至57,711）（MFI中位数为20,995；四分位间距为16,321至29,623）（p<0.001）。我们得出结论，体外循环液中的甲泼尼龙可诱导心脏手术患者单核细胞上血红蛋白清除受体CD163的表达。
