College of Environmental and Resource Sciences, Graduate University of Chinese Academy of Sciences, 100049 Beijing, People's Republic of China.
Can J Microbiol. 2012 Mar;58(3):261-70. doi: 10.1139/w11-129. Epub 2012 Feb 22.
In this study, to give insight into the bacterial diversity of biofilms from full-scale drinking water distribution systems (DWDSs), the bacterial community compositions of biofilms from two urban DWDSs (Guangzhou and Beijing, China) were determined using a 16S rRNA gene library technique. Meanwhile, the occurrence and diversity of mycobacteria were also analyzed by a Mycobacterium -specific hsp gene assay. The biofilms from the full-scale DWDSs have complex bacterial populations. Proteobacteria was the common and predominant group in all biofilm samples, in agreement with previous reports. The community structures of bacteria at the three sites in Guangzhou DWDS were significantly different, despite the similar physicochemical properties of portable water. Some abundant and peculiar bacterial phylotypes were noteworthy, including Methylophilus , Massilia, and Planomicrobium , members of which are rarely found in DWDSs and their roles in DWDS biofilms are still unclear. The diversity of Mycobacterium species in biofilm samples was rather low. Mycobacterium arupense and Mycobacterium gordonae were the primary Mycobacterium species in Guangzhou and Beijing biofilms, respectively, indicating that M. arupense may be more resistant to chloride than M. gordonae.
在这项研究中，为了深入了解饮用水分配系统（DWDS）中生物膜的细菌多样性，我们采用 16S rRNA 基因文库技术，对来自中国两个城市（广州和北京）的两个城市 DWDS 中的生物膜的细菌群落组成进行了测定。同时，还通过分枝杆菌特异性 hsp 基因检测分析了分枝杆菌的发生和多样性。从实际规模的 DWDS 中获得的生物膜具有复杂的细菌种群。变形菌门是所有生物膜样本中常见且主要的菌群，这与以前的报道一致。尽管广州 DWDS 中输水的理化性质相似，但三个地点的细菌群落结构却有显著差异。一些丰富且特殊的细菌类群值得注意，包括甲基杆菌属、马赛菌属和动胶菌属，这些菌属很少在 DWDS 中发现，其在 DWDS 生物膜中的作用尚不清楚。生物膜样本中分枝杆菌的种类多样性较低。格鲁氏分枝杆菌和戈登分枝杆菌分别是广州和北京生物膜中的主要分枝杆菌种类，这表明格鲁氏分枝杆菌可能比戈登分枝杆菌更能耐受氯。
