Allan Wilson Centre for Molecular Ecology and Evolution, School of Biological Sciences, Victoria University of Wellington, Kelburn Parade, Kelburn, PO Box 600 Wellington, New Zealand.
Mol Ecol Resour. 2012 May;12(3):377-88. doi: 10.1111/j.1755-0998.2012.03119.x. Epub 2012 Feb 22.
DNA barcoding has become a well-funded, global enterprise since its proposition as a technique for species identification, delimitation and discovery in 2003. However, the rapid development of next generation sequencing (NGS) has the potential to render DNA barcoding irrelevant because of the speed with which it generates large volumes of genomic data. To avoid obsolescence, the DNA barcoding movement must adapt to use this new technology. This review examines the DNA barcoding enterprise, its continued resistance to improvement and the implications of this on the future of the discipline. We present the consistent failure of DNA barcoding to recognize its limitations and evolve its methodologies, reducing the usefulness of the data produced by the movement and throwing into doubt its ability to embrace NGS.
自 2003 年提出作为物种鉴定、划定和发现的技术以来，DNA 条形码已成为一项资金充足、全球性的事业。然而，下一代测序（NGS）的快速发展有可能使 DNA 条形码变得无关紧要，因为它能够快速生成大量基因组数据。为了避免过时，DNA 条形码运动必须适应新技术的使用。本文回顾了 DNA 条形码事业，其对改进的持续抵制以及这对学科未来的影响。我们提出了 DNA 条形码始终未能认识到其局限性并发展其方法学的问题，从而降低了该运动产生的数据的有用性，并对其接受 NGS 的能力提出了质疑。
