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7年1000次引用之后:针对分类学家和非分类学家的DNA条形码与DNA分类法提议的比较评估

After 7 years and 1000 citations: comparative assessment of the DNA barcoding and the DNA taxonomy proposals for taxonomists and non-taxonomists.

作者信息

Teletchea Fabrice

机构信息

UR AFPA, Domestication in Inland Aquaculture, Nancy Université INRA, Nancy, France.

出版信息

Mitochondrial DNA. 2010 Dec;21(6):206-26. doi: 10.3109/19401736.2010.532212.

Abstract

In 2003, two different approaches-DNA taxonomy and DNA barcoding-were simultaneously proposed to overcome some of the perceived intrinsic weaknesses of the traditional morphology-based taxonomical system, and to help non-taxonomists to resolve their crucial need for accurate and rapid species identification tools. After 7 years, it seems unlikely that a completely new taxonomical system based on molecular characters only (DNA taxonomy) will develop in the future. It is more likely that both morphological and molecular data will be simultaneously analyzed, developing what has been coined as "integrative taxonomy". Concerning DNA barcoding, it is now clear that it does not focus on building a tree-of-life nor to perform DNA taxonomy, but rather to produce a universal molecular identification key based on strong taxonomic knowledge that is collated in the barcode reference library. The indisputable success of the DNA barcoding project is chiefly due to the fact that DNA barcoding standards considerably enhance current practices in the molecular identification field, and standardization offers virtually endless applications for various users.

摘要

2003年,人们同时提出了两种不同的方法——DNA分类学和DNA条形码技术,以克服传统形态学分类系统中一些内在的明显弱点,并帮助非分类学家解决他们对准确、快速的物种识别工具的迫切需求。7年后,未来似乎不太可能仅基于分子特征(DNA分类学)发展出全新的分类系统。更有可能的是,形态学和分子数据将同时进行分析,从而发展出所谓的“综合分类学”。关于DNA条形码技术,现在很清楚的是,它并不专注于构建生命树,也不是进行DNA分类学,而是基于条形码参考库中整理的强大分类学知识,生成一个通用的分子识别密钥。DNA条形码项目无可争议的成功主要归功于这样一个事实,即DNA条形码标准极大地改进了分子识别领域的现有做法,而且标准化为各类用户提供了几乎无限的应用。

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