Liu Yao, Qiu Qiao-meng, Ge Yun, Hong Guang-liang, Lu Zhong-qiu, Liang Huan, Li Meng-fang
Emergercy Medical Department, The First Affiliated Hospital, Wenzhou Medical College, Wenzhou 325000, China.
Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2011 Oct;29(10):735-9.
To observe the effects of hemoperfusion on plasma concentration and histopathological changes in paraquat (PQ) poisoning rabbits.
Sixteen rabbits were randomly divided into exposure group (PQ group, n = 8) and hemoperfusion plus PQ exposure group (HPQ group, n = 8). HPQ group were given hemoperfusion in 45 min after exposure to PQ. The plasma PQ concentrations at 0.5, 1.0, 1.5, 2.0, 3.0, 6.0, 12.0, 24.0, 48.0 and 72.0 hours after exposure were measure in 2 groups. The histopathological changes of lung, liver and kidney were examined, the behavior changes and the survival number of 7 days were observed.
The poisoning symptoms of HPQ group were generally better than those of PQ group, in each group six animals survived for 7d. The plasma PQ concentrations at 1.0, 1.5, 2.0, 3.0, 6.0, 12.0, 24.0, 48.0, 72.0 h after exposure in HPQ group were significantly lower than those in PQ group (P < 0.05 or P < 0.01). In HPQ group, the plasma PQ peak concentration [(5.01 ± 0.15] µg/L], area under the curve [(54.03 ± 5.31) mg×h(-1)×L(-1)] and PQ half-life time [(16.29 ± 3.26) h] after treatment of HP were significantly lower than those [(11.97 ± 0.75) µg/L, (141.40 ± 10.10) mg×h(-1)×L(-1) and (31.16 ± 9.85) h] in PQ group (P < 0.05). The apparent volume of distribution and PQ clearance rate in HPQ group were significantly higher than those in PQ group (P < 0.05). Congestion, edema, cell infiltration and other pathological changes were found in lung, liver and kidney in PQ group under the light microscope, which were significantly more severe than those in HPQ group. The pathologic scores of lung tissue, liver and renal tubular damage on the 1st, 3rd, 7th days after exposure in HPQ group were significantly lower than those in PQ group (P < 0.05).
When acute PQ poising, rabbits appeared the quick absorption, high toxicity and long half-life time of PQ. The early hemoperfusion can effectively remove the toxicant in plasma and reduce the pathological injury in major organs, which may be beneficial for further treatment.
观察血液灌流对百草枯（PQ）中毒家兔血浆浓度及组织病理学变化的影响。
将16只家兔随机分为染毒组（PQ组，n = 8）和血液灌流加PQ染毒组（HPQ组，n = 8）。HPQ组在染毒PQ后45分钟进行血液灌流。分别测定两组染毒后0.5、1.0、1.5、2.0、3.0、6.0、12.0、24.0、48.0和72.0小时的血浆PQ浓度。检查肺、肝和肾的组织病理学变化，观察行为变化及7天存活数。
HPQ组中毒症状总体优于PQ组，每组各有6只动物存活7天。HPQ组染毒后1.0、1.5、2.0、3.0、6.0、12.0、24.0、48.0、72.0小时的血浆PQ浓度显著低于PQ组（P < 0.05或P < 0.01）。HPQ组血液灌流治疗后的血浆PQ峰值浓度[（5.01 ± 0.15）μg/L]、曲线下面积[（54.03 ± 5.31）mg×h⁻¹×L⁻¹]和PQ半衰期[（16.29 ± 3.26）h]均显著低于PQ组[（11.97 ± 0.75）μg/L、（141.40 ± 10.10）mg×h⁻¹×L⁻¹和（31.16 ± 9.85）h]（P < 0.05）。HPQ组的表观分布容积和PQ清除率显著高于PQ组（P < 0.05）。光镜下PQ组肺、肝和肾出现充血、水肿、细胞浸润等病理变化，且明显比HPQ组严重。HPQ组染毒后第1、3、7天肺组织、肝和肾小管损伤的病理评分显著低于PQ组（P < 0.05）。
急性PQ中毒时，家兔PQ吸收迅速、毒性高且半衰期长。早期血液灌流可有效清除血浆中的毒物，减轻主要器官的病理损伤，可能有利于进一步治疗。
