Riahi Reza, Yang Yongliang, Zhang Donna D, Wong Pak Kin
Department of Aerospace and Mechanical Engineering, The University of Arizona, Tucson, AZ, USA.
J Lab Autom. 2012 Feb;17(1):59-65. doi: 10.1177/2211068211426550.
Collective cell migration plays essential roles in a wide spectrum of biological processes, such as embryogenesis, tissue regeneration, and cancer metastasis. Numerous wound-healing assays based on mechanical, chemical, optical, and electrical approaches have been developed to create model "wounds" in cell monolayers to study the collective cell migration processes. These approaches can result in different microenvironments for cells to migrate and possess diverse assay characteristics in terms of simplicity, throughput, reproducibility, and multiplexability. In this review, we provide an overview of advances in wound-healing assays and discuss their advantages and limitations in studying collective cell migration.
集体细胞迁移在广泛的生物过程中起着至关重要的作用，如胚胎发育、组织再生和癌症转移。基于机械、化学、光学和电学方法的众多伤口愈合试验已被开发出来，以在细胞单层中创建模型“伤口”，从而研究集体细胞迁移过程。这些方法会导致细胞迁移的微环境不同，并且在简单性、通量、可重复性和多重性方面具有不同的试验特征。在本综述中，我们概述了伤口愈合试验的进展，并讨论了它们在研究集体细胞迁移方面的优点和局限性。
