Fourth Department of Internal Medicine, Sapporo Medical College, South-1, West-16, 060, Sapporo, Japan.
Cytotechnology. 1989 Feb;2(1):49-57. doi: 10.1007/BF00365414.
In order to establish an efficient culture system for the generation of lymphokine activated killer (LAK) cells, we have developed a new device which is essentially based on a continuous dialyzing culture vessel. LAK cells grown in such a system showed higher cytotoxicity than those grown under conventional culture conditions. By using this new apparatus with continuous regulation of infused interleukin 2, nutritional medium, and pO(2) and pCO(2), yields of 2×10(7) cells/ml were achieved and maintained for more than 21 days. These cells also showed a significant increase of LAK activity on a per cell basis.
为了建立一种高效的淋巴因子激活的杀伤（LAK）细胞培养体系，我们开发了一种新装置，该装置主要基于连续透析培养容器。在这种系统中生长的 LAK 细胞比在传统培养条件下生长的细胞具有更高的细胞毒性。通过使用这种新装置，连续调节输注的白细胞介素 2、营养培养基以及 pO2 和 pCO2，可以实现 2×107 个细胞/ml 的产率，并维持超过 21 天。这些细胞在单细胞基础上也显示出 LAK 活性的显著增加。
