Institut für Biochemie, Westfälische Wilhelms-Universität, Wilhelm-Klemm-Straße 2, D-48149, Münster, F.R, Germany.
Cytotechnology. 1997 May;24(1):11-7. doi: 10.1023/A:1007936323956.
Astrocytes, a member of the glial cell family in the central nervous system, are assumed to play a crucial role in the formation of the blood-brain barrier (BBB) in vertebrates. It was shown that astrocytes induce BBB-properties in brain capillary endothelial cells (BCEC) in vitro. We now established an astroglial cell line of non-tumoral origin. The cloned cell line (A7) shows a highly increased proliferation rate and expresses the astrocytic marker glial fibrillary acidic protein. Furthermore, the clone A7 expresses S-100-protein and vimentin, which are also expressed by primary cultured astrocytes. This cell line therefore shows general astrocytic features. In addition, we were able to show that A7 cells re-induce the BBB-related marker enzyme alkaline phosphatase in BCEC, when these two cell types are co-cultured. Thus we have a cell line which can be readily cultured in large quantities, shows common astrocyte properties and is able to influence BCEC with respect to a BBB-related feature.
星形胶质细胞是中枢神经系统中神经胶质细胞的一个成员，被认为在脊椎动物血脑屏障 (BBB) 的形成中发挥着关键作用。研究表明，星形胶质细胞在体外诱导脑毛细血管内皮细胞 (BCEC) 形成 BBB 特性。我们现在建立了一个非肿瘤起源的星形胶质细胞系。克隆细胞系 (A7) 表现出高度增加的增殖率，并表达星形胶质细胞标志物胶质纤维酸性蛋白。此外，该克隆 A7 表达 S-100 蛋白和波形蛋白，这两种蛋白也由原代培养的星形胶质细胞表达。因此，该细胞系表现出一般的星形胶质细胞特征。此外，当这两种细胞类型共培养时，我们能够证明 A7 细胞在 BCEC 中重新诱导与 BBB 相关的标记酶碱性磷酸酶。因此，我们有一个可以大量容易培养的细胞系，具有常见的星形胶质细胞特性，并能够影响 BCEC 的 BBB 相关特征。
