Department of Biology and Chemistry, City University of Hong Kong, Kowloon, Hongkong.
Cytotechnology. 1997 May;24(1):47-54. doi: 10.1023/A:1007914004727.
To increase the yield of monoclonal antibody in a hybridoma culture, it is important to optimize the combination of several factors including cell density, antibody productivity per cell, and the duration of the culture. Potassium acetate enhances the production of antibodies by cells but sometimes depresses cell density. The production of anti-(human B-type red blood cell surface antigen) antibody by Cp9B hybridoma was studied. In batch cultures, potassium acetate inhibited Cp9B cells growth and decreased the maximal cell density but the productivity of antibody per cell was increased. The balance of the two effects resulted in a slight decline of antibody production. In a stirred tank bioreactor, the inhibitory effect of potassium acetate on cell density was overcome by applying the perfusion technique with the attachment of a cell-recycling apparatus to the bioreactor. In such a reactor, potassium acetate at 1 g l(-1) did not cause a decrease in the cell density, and the antibody concentration in the culture supernatant was increased from 28 μg ml(-1) to 38 μg ml(-1). Potassium acetate also suppressed the consumption of glucose and the accumulation of lactate in batch cultures, but the glucose and lactate levels were kept stable by applying the perfusion technique in the stirred tank bioreactor.
为了提高杂交瘤培养物中单克隆抗体的产量，优化细胞密度、每个细胞的抗体产量和培养时间等几个因素的组合非常重要。醋酸钾可以增强细胞产生抗体的能力，但有时会降低细胞密度。本研究以 Cp9B 杂交瘤为例，探讨了醋酸钾对抗体产生的影响。在分批培养中，醋酸钾抑制 Cp9B 细胞生长，降低最大细胞密度，但增加了每个细胞的抗体产量。这两种效应的平衡导致抗体产量略有下降。在搅拌罐生物反应器中，通过应用灌注技术并在生物反应器上附加细胞回收装置，可以克服醋酸钾对细胞密度的抑制作用。在这种反应器中，1 g/L 的醋酸钾不会降低细胞密度，并且培养上清液中的抗体浓度从 28μg/ml 增加到 38μg/ml。醋酸钾还抑制了分批培养中葡萄糖的消耗和乳酸的积累，但通过在搅拌罐生物反应器中应用灌注技术，可以使葡萄糖和乳酸水平保持稳定。
