Croix Rouge Canadienne, Centre de Québec, Sainte-Foy, P. Q., Canada.
Biotechnol Bioeng. 1991 Oct 5;38(7):781-7. doi: 10.1002/bit.260380712.
In order to elucidate the hybridoma life cycle and the limiting factors in perfusion systems, we performed cultures in a stirred tank bioreactor, coupled to an external tangential flow filtration unit. Cell density and antibody production in perfusion were consistent with previous studies. The average life span of the cells (2.1-2.2 days), antibody, productivity per cell produced (30-38 mg/10(9) cells) and cell size diameter evolution appeared similar to values observed in batch cultures. These observations highly suggest a similar "grow or die" life cycle. Cell and antibody production, strictly related to the medium perfusion rate, seem to be under the control of the nutrient availability. A hypothesis to explain such a life cycle of hybridoma cells in perfusion systems and a model for viable and dead cell density is proposed.
为了阐明杂交瘤细胞的生命循环和灌注系统中的限制因素，我们在搅拌槽生物反应器中进行培养，该反应器与外部切向流过滤单元相耦合。灌注培养中的细胞密度和抗体产量与之前的研究一致。细胞的平均寿命（2.1-2.2 天）、抗体产量、每个细胞产生的抗体生产力（30-38mg/10(9)个细胞）和细胞大小直径的演变似乎与批次培养中观察到的值相似。这些观察结果高度表明存在类似的“生长或死亡”的生命循环。细胞和抗体的产生与培养基灌注率密切相关，似乎受到营养物质可用性的控制。提出了一种解释灌注系统中杂交瘤细胞这种生命循环的假说，以及一个用于活细胞和死细胞密度的模型。
