Hoff H F, Karagas M, Heideman C L, Gaubatz J W, Gotto A M
Atherosclerosis. 1979 Mar;32(3):259-68. doi: 10.1016/0021-9150(79)90169-2.
The amounts of buffer- and Triton-extracted apo B (LDL-protein), as well as the sum of these two fractions, were correlated with the total tissue cholesterol and hydroxyproline content (as a measure of collagen) in grossly normal intima, fatty streaks, and fibrous plaques of human aortas obtained at autopsy. Quantitative values of buffer- and Triton-extracted apo B were obtained by sequentially extracting homogenates of aortic intima with an aqueous buffer and one containing Triton X-100, and measuring the apo B content in each extract by an electroimmunoassay relative to plasma LDL or Triton-treated LDL. Significant positive correlations were obtained between the following: tissue cholesterol and both buffer-extracted and total-extracted apo B in grossly normal intima; tissue cholesterol and Triton-extracted apo B in microdissected fibrotic caps and cores of fibrous plaques, as well as in whole plaques. A positive correlation was also obtained between tissue cholesterol and total-extracted apo B in the necrotic core. A significant negative correlation was found between Triton-extracted apo B and collagen in whole plaques. The calculated mean percent of total tissue cholesterol in the different aortic regions that could be present as part of an intact LDL particle were: 100% in grossly normal intima, 16% in fatty streaks, and 11% in fibrous plaques. The positive correlation between Triton-extracted apo B and cholesterol in plaques suggests one or both of the following: the extracellular pool of cholesterol or some material increasing concurrently with cholesterol interacts with apo B or another part of the LDL particle; or the apo B containing lipoprotein is trapped in the hydrophobic environment of extracellular lipid. Both possibilities would render the particle less soluble in aqueous buffers. The negative correlation between Triton-extracted apo B and tissue collagen and the lack of a significant correlation between buffer-extracted apo B and collagen content suggests that collagen is probably not responsible for apo B retention in the aortic intima.
在尸检获得的人类主动脉大体正常内膜、脂纹和纤维斑块中，缓冲液和 Triton 提取的载脂蛋白 B（低密度脂蛋白蛋白）含量，以及这两个组分的总和，与总组织胆固醇和羟脯氨酸含量（作为胶原蛋白的指标）相关。通过依次用缓冲水溶液和含 Triton X - 100 的溶液提取主动脉内膜匀浆，并通过相对于血浆低密度脂蛋白或 Triton 处理的低密度脂蛋白的免疫电泳法测量各提取物中的载脂蛋白 B 含量，获得缓冲液和 Triton 提取的载脂蛋白 B 的定量值。在以下各项之间获得了显著正相关：大体正常内膜中的组织胆固醇与缓冲液提取的和总提取的载脂蛋白 B；纤维斑块的显微切割纤维化帽和核心以及整个斑块中的组织胆固醇与 Triton 提取的载脂蛋白 B。在坏死核心中，组织胆固醇与总提取的载脂蛋白 B 之间也获得了正相关。在整个斑块中，发现 Triton 提取的载脂蛋白 B 与胶原蛋白之间存在显著负相关。在不同主动脉区域中，可作为完整低密度脂蛋白颗粒一部分存在的总组织胆固醇的计算平均百分比为：大体正常内膜中为 100%，脂纹中为 16%，纤维斑块中为 11%。斑块中 Triton 提取的载脂蛋白 B 与胆固醇之间的正相关表明以下一种或两种情况：胆固醇的细胞外池或与胆固醇同时增加的某些物质与载脂蛋白 B 或低密度脂蛋白颗粒的另一部分相互作用；或者含载脂蛋白 B 的脂蛋白被困在细胞外脂质的疏水环境中。这两种可能性都会使颗粒在水性缓冲液中的溶解度降低。Triton 提取的载脂蛋白 B 与组织胶原蛋白之间的负相关以及缓冲液提取的载脂蛋白 B 与胶原蛋白含量之间缺乏显著相关性表明，胶原蛋白可能不是主动脉内膜中载脂蛋白 B 滞留的原因。
