重组人白蛋白在细胞培养中的应用：体外评价对 NRK 和 SCC-9 细胞的促生长潜力。
Recombinant Human Albumin in Cell Culture: Evaluation of Growth-Promoting Potential for NRK and SCC-9 Cells In Vitro.
出版信息
Cytotechnology. 1997 Sep;24(3):243-52. doi: 10.1023/A:1007916930200.
Serum-derived albumin has for a long time been used in cell culture media, but the exact role of albumin and/or impurities bound to albumin has not been precisely defined. In this study, recombinant human albumin was evaluated for its growth-promoting activity on two cell lines, NRK and SCC-9. For NRK cells, the recombinant human albumin was found to exert an inhibitory effect. The fact that fatty acid free HSA was also inhibitory while HSA fraction V was stimulatory suggested a role for fatty acids or some other bound moieties in growth stimulation by HSA fraction V. Addition of oleic acid, cholesterol, phosphatidylcholine, phosphatidylserine or a combination of these lipids, however, did not significantly improve the growth stimulating activity of either fatty acid free HSA or the recombinant human albumin. For SCC-9 cells, both recombinant human albumin and fatty acid free HSA showed slight stimulation (although they were not as active as HSA fraction V), suggesting that in some cell systems, the albumin molecule per se may promote cell growth and survival.
血清白蛋白长期以来一直被用于细胞培养基中，但白蛋白的确切作用以及与白蛋白结合的杂质的作用尚未得到明确界定。在这项研究中，评估了重组人白蛋白对两种细胞系 NRK 和 SCC-9 的促生长活性。对于 NRK 细胞，发现重组人白蛋白具有抑制作用。事实上，游离脂肪酸的 HSA 也是抑制性的，而 HSA 分馏 V 是刺激性的，这表明脂肪酸或 HSA 分馏 V 中生长刺激的其他一些结合部分在生长刺激中起作用。然而，添加油酸、胆固醇、磷脂酰胆碱、磷脂酰丝氨酸或这些脂质的组合并没有显著提高游离脂肪酸的 HSA 或重组人白蛋白的生长刺激活性。对于 SCC-9 细胞，重组人白蛋白和游离脂肪酸的 HSA 均显示出轻微的刺激作用（尽管它们不如 HSA 分馏 V 活跃），这表明在某些细胞系统中，白蛋白分子本身可能促进细胞生长和存活。
Zotero 插件