Cytotechnology. 1997 Nov;25(1-3):9-15. doi: 10.1023/A:1007950002663.
An adaptive fuzzy controller was developed to control the glucose and glutamine concentrations in the reactor constant at the desired level. The parameter values of the controller change during the cultivation according to the culture phase which was detected by the lactate concentration. Cultivations with different glucose and glutamine set point concentrations of a recombinant BHK anchorage-dependent cell line were performed in a fed-batch reactor on-line connected with an HPLC system. Glucose and glutamine concentrations were satisfactorily controlled at each set point during all cultivation periods. Ammonia had a determining effect on productivity since it inhibited cell growth and protein specific production. Ammonia production increased with an increase of glutamine or a decrease of glucose set point concentrations, indicating the importance of glucose to glutamine ratio for the optimization of productivity in mammalian cell cultures.
开发了一种自适应模糊控制器，以将反应器中的葡萄糖和谷氨酰胺浓度控制在所需水平。根据通过乳酸浓度检测到的培养阶段，控制器的参数值在培养过程中发生变化。在与 HPLC 系统在线连接的分批进料式反应器中，对重组 BHK 贴壁依赖性细胞系进行了不同葡萄糖和谷氨酰胺设定点浓度的培养。在所有培养期间，葡萄糖和谷氨酰胺浓度都能令人满意地控制在每个设定点。氨对生产力有决定性影响，因为它抑制细胞生长和蛋白质特异性生产。随着谷氨酰胺或葡萄糖设定点浓度的增加或降低，氨的产量增加，表明葡萄糖与谷氨酰胺的比值对优化哺乳动物细胞培养中的生产力非常重要。
